An UPLC-MS-based metabolomics investigation on the anti-fatigue effect of salidroside in mice

J Pharm Biomed Anal. 2015 Feb;105:84-90. doi: 10.1016/j.jpba.2014.11.036. Epub 2014 Dec 4.


An ultra-performance liquid chromatography-quadrupole time-of-flight-based metabolomic approach was developed to study influence of salidroside, an anti-fatigue ingredient from Rhoiola rosea, on urinary metabolic profiling of rats to a single dose of 180 mg/kg per day. Unsupervised principal component analysis (PCA) and supervised orthogonal pre-projection to latent structures discriminate analysis (OPLS-DA) on metabolite profiling revealed obvious differentiation between the salidroside treated groups and controls in both positive and negative ion modes. Eleven urinary metabolites contributing to the differentiation were identified as anti-fatigue biomarkers: N-acetylserotonin, 2-Methoxyestrone 3-glucuronide, Taurine, Melatonin, Sorbitol, Geranyl diphosphate, Z-nucleotide, Cortisone, Dihydrocortisol, Sebacic acid, Pregnenolone sulfate. The physiological significance of these biomarkers is discussed. The work showed that metabolomics is a powerful tool in studying the anti-fatigue effects of natural compound salidroside on multiple targets in vivo.

Keywords: Anti-fatigue; Biomarkers; Metabolomics; Salidroside; UPLC-MS/MS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / urine
  • Chromatography, High Pressure Liquid / instrumentation
  • Chromatography, High Pressure Liquid / methods*
  • Fatigue / drug therapy*
  • Fatigue / urine
  • Glucosides / isolation & purification
  • Glucosides / urine*
  • Male
  • Metabolomics / instrumentation
  • Metabolomics / methods*
  • Mice, Inbred Strains
  • Multivariate Analysis
  • Phenols / isolation & purification
  • Phenols / urine*
  • Principal Component Analysis
  • Rats
  • Rhodiola / chemistry
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Swimming


  • Biomarkers
  • Glucosides
  • Phenols
  • rhodioloside