Negative enrichment of bovine T lymphocytes with monoclonal antibodies and magnetic microspheres

Vet Immunol Immunopathol. 1989 Sep;22(2):161-73. doi: 10.1016/0165-2427(89)90058-5.


A highly enriched population of bovine T lymphocytes was produced from peripheral blood leukocytes following the depletion of monoclonal antibody-labelled B lymphocytes and monocytes with magnetic microspheres. This negative-enrichment protocol was simple, rapid, and specific. Also, it had a high recovery efficiency and was consistently reproducible. The enriched T lymphocytes proliferated in response to recombinant bovine interleukin 2 and, following the addition of monocytes, to concanavalin A. This methodology made it possible to determine the proliferative responses of peripheral blood lymphocytes utilizing a constant number of T lymphocytes within each assay. In this way, the in vitro T lymphocyte responses were determined independent of changes in the number of responder cells within peripheral blood.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • B-Lymphocytes / immunology
  • Cattle
  • Cell Separation / methods*
  • Concanavalin A / pharmacology
  • Flow Cytometry
  • Herpesvirus 1, Bovine / immunology
  • Infectious Bovine Rhinotracheitis / immunology
  • Interleukin-2 / pharmacology
  • Lymphocyte Activation
  • Magnetics
  • Microspheres
  • Monocytes / immunology
  • Recombinant Proteins
  • T-Lymphocytes* / immunology
  • T-Lymphocytes* / metabolism


  • Antibodies, Monoclonal
  • Interleukin-2
  • Recombinant Proteins
  • Concanavalin A