Interneuron activity leads to initiation of low-voltage fast-onset seizures

Ann Neurol. 2015 Mar;77(3):541-6. doi: 10.1002/ana.24342. Epub 2015 Feb 14.


Seizures in temporal lobe epilepsy can be classified as hypersynchronous and low-voltage fast according to their onset patterns. Experimental evidence suggests that low-voltage fast-onset seizures mainly result from the synchronous activity of γ-aminobutyric acid-releasing cells. In this study, we tested this hypothesis using the optogenetic control of parvalbumin-positive interneurons in the entorhinal cortex, in the in vitro 4-aminopyridine model. We found that both spontaneous and optogenetically induced seizures had similar low-voltage fast-onset patterns. In addition, both types of seizures presented with higher ripple than fast ripple rates. Our data demonstrate the involvement of interneuronal networks in the initiation of low-voltage fast-onset seizures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 4-Aminopyridine / pharmacology
  • Animals
  • Electrophysiological Phenomena / physiology*
  • Entorhinal Cortex / cytology
  • Entorhinal Cortex / physiopathology*
  • GABAergic Neurons / cytology
  • GABAergic Neurons / physiology*
  • Interneurons / cytology
  • Interneurons / physiology*
  • Mice
  • Nerve Net / cytology
  • Nerve Net / physiopathology
  • Optogenetics
  • Parvalbumins / metabolism
  • Patch-Clamp Techniques
  • Seizures / physiopathology*
  • gamma-Aminobutyric Acid / metabolism


  • Parvalbumins
  • gamma-Aminobutyric Acid
  • 4-Aminopyridine