A novel erythromycin resistance plasmid from Bacillus sp. strain HS24, isolated from the marine sponge Haliclona simulans

PLoS One. 2014 Dec 30;9(12):e115583. doi: 10.1371/journal.pone.0115583. eCollection 2014.


A better understanding of the origin and natural reservoirs of resistance determinants is fundamental to efficiently tackle antibiotic resistance. This paper reports the identification of a novel 5.8 kb erythromycin resistance plasmid, from Bacillus sp. HS24 isolated from the marine sponge Haliclona simulans. pBHS24B has a mosaic structure and carries the erythromycin resistance gene erm(T). This is the first report of an erythromycin resistance plasmid from a sponge associated bacteria and of the Erm(T) determinant in the genus Bacillus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aquatic Organisms / microbiology*
  • Bacillus / genetics*
  • Base Sequence
  • Drug Resistance, Bacterial / genetics*
  • Erythromycin*
  • Molecular Sequence Data
  • Plasmids / genetics*
  • Porifera / microbiology*


  • Erythromycin

Associated data

  • GENBANK/JF803858
  • GENBANK/KC991136

Grants and funding

This research was supported in parts by grants awarded to FOG by the Science Foundation of Ireland (SSPC2 12/RC/2275, 13-TIDA-B2625, 07/IN.1/B948, 12/TIDA/B2411, 12/TIDA/B2405,09/RFP/BMT 2350); the Department of Agriculture, Fisheries and Food (DAFF11/F/009 MabS, FIRM/RSF/CoFoRD; FIRM 08/RDC/629); the Environmental Protection Agency (EPA 2008-PhD/S-2), the Irish Research Council for Science, Engineering and Technology (PD/2011/2414; RS/2010/2413), the European Commission (FP7-PEOPLE-2013-ITN, 607786; OCEAN2012, 287589; FP7-KBBE-2012-6, CP-TP 311975; FP7-KBBE-2012-6, CP-TP-312184; Marie Curie 256596); and the Marine Institute (Beaufort award C2CRA 2007/082); Teagasc (Walsh Fellowship 2013) and the Health Research Board (HRA/2009/146). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.