Analysis of serum cytokine levels in larynx squamous cell carcinoma and dysplasia patients

Iran J Immunol. 2014 Dec;11(4):259-68.


Background: Although the imbalance of cytokines in Head and Neck Squamous Cell Carcinoma (HNSCC) is well known, there is scarce data regarding its occurrence during dysplasia, before the malignant transformation.

Objective: To determine whether laryngeal dysplasia patients show a different cytokine profile than patients with cancer and healthy controls.

Methods: Seventeen newly diagnosed, untreated larynx squamous cell carcinoma (SCC) and six laryngeal dysplasia patients as well as 22 healthy controls were analyzed for circulating cytokines. A flowcytometry Th1/Th2 cytokine array kit was used to quantitatively measure Interleukin-2 (IL-2), IL-4, IL-6, IL-10, Tumor Necrosis Factor-α (TNF-α) and Interferon-γ (IFN-γ) levels. Additionally, IL-8 levels were determined through ELISA.

Results: IL-6, IL-8 and IL-10 were determined to be statistically increased in SCC patients (p<0.05). IL-8 and IL-10 levels were also higher in SCC patients than dysplasia patients (p<0.05). Additionally, IL-6 and IL-10 were all found to be markedly increased in dysplasia patients compared with controls (p<0.05).

Conclusion: Our results demonstrate an imbalance of IL-6 and IL-10 not only in HNSCC but also in laryngeal dysplasia.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Carcinoma, Squamous Cell / immunology*
  • Cytokines / metabolism*
  • Female
  • Humans
  • Laryngeal Diseases / immunology*
  • Laryngeal Neoplasms / immunology*
  • Larynx / pathology*
  • Male
  • Middle Aged
  • Precancerous Conditions / immunology*
  • Th2 Cells / immunology*
  • Young Adult


  • Cytokines