Olive oil and vitamin D synergistically prevent bone loss in mice

PLoS One. 2014 Dec 31;9(12):e115817. doi: 10.1371/journal.pone.0115817. eCollection 2014.


As the Mediterranean diet (and particularly olive oil) has been associated with bone health, we investigated the impact of extra virgin oil as a source of polyphenols on bone metabolism. In that purpose sham-operated (SH) or ovariectomized (OVX) mice were subjected to refined or virgin olive oil. Two supplementary OVX groups were given either refined or virgin olive oil fortified with vitamin D3, to assess the possible synergistic effects with another liposoluble nutrient. After 30 days of exposure, bone mineral density and gene expression were evaluated. Consistent with previous data, ovariectomy was associated with increased bone turnover and led to impaired bone mass and micro-architecture. The expression of oxidative stress markers were enhanced as well. Virgin olive oil fortified with vitamin D3 prevented such changes in terms of both bone remodeling and bone mineral density. The expression of inflammation and oxidative stress mRNA was also lower in this group. Overall, our data suggest a protective impact of virgin olive oil as a source of polyphenols in addition to vitamin D3 on bone metabolism through improvement of oxidative stress and inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Composition / drug effects
  • Bone Demineralization, Pathologic / prevention & control*
  • Bone Density / drug effects*
  • Bone Density Conservation Agents / therapeutic use
  • Bone and Bones / metabolism
  • Bone and Bones / pathology
  • Diet, Mediterranean
  • Dietary Fats, Unsaturated / therapeutic use
  • Drug Synergism
  • Estrogens / deficiency
  • Female
  • Mice
  • Mice, Inbred C57BL
  • Olive Oil
  • Ovariectomy
  • Oxidative Stress / drug effects
  • Plant Oils / therapeutic use*
  • Polyphenols / metabolism
  • Vitamin D / therapeutic use*


  • Bone Density Conservation Agents
  • Dietary Fats, Unsaturated
  • Estrogens
  • Olive Oil
  • Plant Oils
  • Polyphenols
  • Vitamin D

Grant support

CT was funded by a Conventions Industrielles de Formation par la REcherche grant (CIFRE; n° 2011/99) from the French National Association for Research and Technology (http://www.anrt.asso.fr/) in partnership with Lesieur. This work was supported by both the CIFRE grant and the pOLIVd3 ANR (French National Research Agency; http://www.agence-nationale-recherche.fr/) program ANR-10-ALIA-0008. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.