Effect of Ginkgo biloba on brain volume after carotid artery occlusion in rats: a stereological and histopathological study

Turk J Med Sci. 2014;44(4):546-53. doi: 10.3906/sag-1305-40.


Background/aim: This study investigated the effect of Ginkgo biloba (GB) on brain volume in cerebral ischemia induced by stopping carotid artery blood flow.

Materials and methods: Twenty-four adult male rats were divided into 4 groups of 6 rats each. No procedure was performed on the control group. Ischemia was applied to the rats in the ischemia and ischemia + GB groups by clamping the arteria carotis communis for 30 min. The rats in the ischemia + GB group were given 100 mg/kg drops (Tebokan Fort Drop, Abdi Ibrahim Ilaç Sanayi A.$., Turkey) containing dry GB leaf extract orally, every day for 14 days from the day of ischemia. In the sham group, surgical stress alone was applied by performing a skin incision. On the 14th day, brain tissues were extracted and evaluated stereologically and histopathologically.

Results: The only statistically significant difference was observed between the sham and control groups.

Conclusion: This result may be interpreted as surgical stress, established by cutaneous incision, having an adverse effect on brain volume. Additionally, the absence of any difference in terms of brain volume following 30 min of ischemia between the ischemia and control groups suggests that a probable postischemic rise in brain volume disappears within 14 days.

MeSH terms

  • Animals
  • Arterial Occlusive Diseases / complications
  • Arterial Occlusive Diseases / drug therapy*
  • Arterial Occlusive Diseases / pathology
  • Brain Ischemia / drug therapy*
  • Brain Ischemia / etiology
  • Brain Ischemia / pathology
  • Carotid Artery Diseases / complications
  • Carotid Artery Diseases / drug therapy*
  • Carotid Artery Diseases / pathology
  • Disease Models, Animal
  • Ginkgo biloba
  • Male
  • Organ Size
  • Phytotherapy*
  • Plant Extracts / therapeutic use*
  • Rats
  • Rats, Sprague-Dawley


  • Plant Extracts
  • Ginkgo biloba extract