Abstract
We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, 37% fewer RT-PCR tests are needed.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteria / classification*
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Bacteria / genetics
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Bacteria / isolation & purification*
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Costs and Cost Analysis
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DNA, Bacterial / genetics*
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DNA, Bacterial / isolation & purification
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Freeze Drying / economics
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Freeze Drying / methods
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Humans
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Nasopharynx / microbiology*
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Real-Time Polymerase Chain Reaction / economics
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Real-Time Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Specimen Handling / economics
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Specimen Handling / methods*