Effects of margarine enriched with plant sterol esters from rapeseed and tall oils on markers of endothelial function, inflammation and hemostasis

Scand J Clin Lab Invest. 2015 Apr;75(2):189-92. doi: 10.3109/00365513.2014.992040. Epub 2015 Jan 1.

Abstract

Background and aims: The sterol profile of rapeseed oil differs from that of tall oil with higher contents of campesterol and brassicasterol. We previously found that margarines providing 2 g/day of sterols from rapeseed or tall oil resulted in similar reductions in LDL cholesterol of 8-9%. The aim of the present study was to investigate whether the consumption of these margarines affected markers of endothelial function, inflammation and hemostasis.

Methods: Blood samples were collected from 58 hypercholesterolemic volunteers who completed a double-blinded, randomized, crossover trial. Subjects consumed each of the two sterol margarines and a control non-sterol margarine for 4 weeks separated by one-week washout periods. All the margarines had the same fatty acid composition. Concentrations of vascular cell adhesion molecule-l (VCAM-1), E-selection, circulating tumor necrosis factor α (TNFα) and plasminogen activator inhibitor-1 (total, tPAI-1; active, PAI-1) were quantified.

Results: Rapeseed-sterol margarine reduced E-selection concentrations compared to the control margarine (p = 0.012) while tall-sterol margarine had no effect. The rapeseed-sterol margarine also reduced tPAI-1 (p = 0.008) compared to the tall-sterol margarine. No significant changes were observed in TNFα and VCAM-1. No association was found between LDL reduction and changes in E-selection and tPAI-1.

Conclusion: Rapeseed-sterol margarine demonstrated favorable effects on vascular risk markers.

Keywords: Cardiovascular disease; E-selectin; inflammation; margarine; plant sterols; plasminogen activator inhibitor.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Aged
  • Biomarkers / blood
  • Brassica rapa / chemistry
  • Cholestadienols / pharmacology
  • Cholesterol, LDL / blood
  • Cytokines / blood
  • E-Selectin / blood
  • Endothelium, Vascular / drug effects*
  • Endothelium, Vascular / physiology
  • Female
  • Hemostasis / drug effects
  • Humans
  • Hypercholesterolemia / diet therapy*
  • Inflammation / blood
  • Inflammation / prevention & control
  • Male
  • Margarine*
  • Middle Aged
  • Phytosterols / chemistry
  • Phytosterols / pharmacology*
  • Plant Oils / chemistry
  • Vascular Cell Adhesion Molecule-1 / blood

Substances

  • Biomarkers
  • Cholestadienols
  • Cholesterol, LDL
  • Cytokines
  • E-Selectin
  • Phytosterols
  • Plant Oils
  • SELE protein, human
  • Vascular Cell Adhesion Molecule-1
  • tall oil
  • brassicasterol
  • Margarine