OsGL1-3 is involved in cuticular wax biosynthesis and tolerance to water deficit in rice

PLoS One. 2015 Jan 2;10(1):e116676. doi: 10.1371/journal.pone.0116676. eCollection 2015.

Abstract

Cuticular wax covers aerial organs of plants and functions as the outermost barrier against non-stomatal water loss. We reported here the functional characterization of the Glossy1(GL1)-homologous gene OsGL1-3 in rice using overexpression and RNAi transgenic rice plants. OsGL1-3 gene was ubiquitously expressed at different level in rice plants except root and its expression was up-regulated under ABA and PEG treatments. The transient expression of OsGL1-3-GFP fusion protein indicated that OsGL1-3 is mainly localized in the plasma membrane. Compared to the wild type, overexpression rice plants exhibited stunted growth, more wax crystallization on leaf surface, and significantly increased total cuticular wax load due to the prominent changes of C30-C32 aldehydes and C30 primary alcohols. While the RNAi knockdown mutant of OsGL1-3 exhibited no significant difference in plant height, but less wax crystallization and decreased total cuticular wax accumulation on leaf surface. All these evidences, together with the effects of OsGL1-3 on the expression of some wax synthesis related genes, suggest that OsGL1-3 is involved in cuticular wax biosynthesis. Overexpression of OsGL1-3 decreased chlorophyll leaching and water loss rate whereas increased tolerance to water deficit at both seedling and late-tillering stages, suggesting an important role of OsGL1-3 in drought tolerance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / pharmacology
  • Adaptation, Physiological*
  • Cell Membrane Permeability
  • Chlorophyll / metabolism
  • Droughts
  • Gene Expression Regulation, Plant / drug effects
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Microscopy, Electron, Scanning
  • Oryza / genetics
  • Oryza / metabolism*
  • Phylogeny
  • Plant Epidermis / genetics
  • Plant Epidermis / metabolism
  • Plant Growth Regulators / pharmacology
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Plant Leaves / ultrastructure
  • Plant Proteins / classification
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Plants, Genetically Modified
  • RNA Interference
  • Reverse Transcriptase Polymerase Chain Reaction
  • Seedlings / genetics
  • Seedlings / metabolism
  • Water / metabolism*
  • Waxes / metabolism*

Substances

  • Membrane Proteins
  • Plant Growth Regulators
  • Plant Proteins
  • Waxes
  • Water
  • Chlorophyll
  • Abscisic Acid

Grant support

This study was supported by Specialized Research Fund for the Doctoral Program of Higher Education of China (20124320110012), Open Research Fund of the State Key Laboratory of Main Crop Germplasm Innovation, Key Science and Technology Project of Hunan Province (2014WK2004), and Construct Program of the Key Discipline in Hunan Province. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.