Inositol 1,4,5-trisphosphate receptors in the endoplasmic reticulum: A single-channel point of view

Cell Calcium. 2015 Jul;58(1):67-78. doi: 10.1016/j.ceca.2014.12.008. Epub 2014 Dec 18.


As an intracellular Ca(2+) release channel at the endoplasmic reticulum membrane, the ubiquitous inositol 1,4,5-trisphosphate (InsP3) receptor (InsP3R) plays a crucial role in the generation, propagation and regulation of intracellular Ca(2+) signals that regulate numerous physiological and pathophysiological processes. This review provides a concise account of the fundamental single-channel properties of the InsP3R channel: its conductance properties and its regulation by InsP3 and Ca(2+), its physiological ligands, studied using nuclear patch clamp electrophysiology.

Keywords: Calcium signal; Endoplasmic reticulum; Inositol 1,4,5-trisphosphate receptor; Intracellular calcium; Ion channel; Patch clamp electrophysiology.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Calcium / metabolism
  • Calcium Signaling
  • Endoplasmic Reticulum / metabolism*
  • Humans
  • Inositol 1,4,5-Trisphosphate / metabolism
  • Inositol 1,4,5-Trisphosphate Receptors / genetics
  • Inositol 1,4,5-Trisphosphate Receptors / metabolism*
  • Ion Channel Gating
  • Patch-Clamp Techniques


  • Inositol 1,4,5-Trisphosphate Receptors
  • Inositol 1,4,5-Trisphosphate
  • Calcium