Effect of high-fat or high-glucose diet on obesity and visceral adipose tissue in mice

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2014 Dec;36(6):614-9. doi: 10.3881/j.issn.1000-503X.2014.06.010.


Objective: To investigate the effect of high-fat or high-glucose diet on obesity and visceral adipose tissue in C57BL/6 mice.

Methods: Four-week-old C57BL/6 mice were allocated into normal diet group,high-fat diet group,and high-glucose diet group according to the random number table until 20 weeks old. Body weight,epididymal adipose tissue weight,blood leptin,fat infiltration in liver,M1/M2 macrophage subtypes,and monocyte chemoattractant protein-1 mRNA in epididymal adipose tissues were measured.

Results: Compared with normal diet group,body weight,epididymal adipose tissue weight,and leptin concentration in high fat diet group at 20 weeks were significantly increased (P < 0.05),and oil red O staining showed more prominent adipocyte infiltration in liver in high-fat diet group than those in normal diet and high-glucose diet group. However,no apparent differences were seen in high-glucose diet group at 20 weeks in terms of body weight,epididymal adipose tissue weight and leptin concentration. In high-fat diet group,the macrophages infiltration in epididymal adipose tissue increased with time and the percentage of M2 macrophage decreased in high-fat diet group than that in high-glucose diet group(P<0.05). Compared with normal diet group,monocyte chemoattractant protein-1 mRNA expression increased significantly in high-fat diet group(P<0.05). In high-glucose group,however,no significant differences were discerned (P > 0.05).

Conclusion: High-fat diet,rather than 60% high glucose diet,will lead to obesity and macrophage infiltration in adipose tissues.

MeSH terms

  • Adipocytes
  • Adipose Tissue
  • Animals
  • Body Weight
  • Chemokine CCL2 / genetics
  • Chemokine CCL2 / metabolism
  • Diet, High-Fat / methods*
  • Glucose / administration & dosage
  • Intra-Abdominal Fat*
  • Leptin
  • Macrophages
  • Mice
  • Mice, Inbred C57BL
  • Obesity*
  • RNA, Messenger


  • Chemokine CCL2
  • Leptin
  • RNA, Messenger
  • Glucose