Differential distribution of GABAA receptor mRNAs in bovine cerebellum--localization of alpha 2 mRNA in Bergmann glia layer

W Wisden; L A McNaughton; M G Darlison; S P Hunt; E A Barnard

doi:10.1016/0304-3940(89)90193-6

Differential distribution of GABAA receptor mRNAs in bovine cerebellum--localization of alpha 2 mRNA in Bergmann glia layer

Neurosci Lett. 1989 Nov 20;106(1-2):7-12. doi: 10.1016/0304-3940(89)90193-6.

Authors

W Wisden¹, L A McNaughton, M G Darlison, S P Hunt, E A Barnard

Affiliation

¹ MRC Molecular Neurobiology Unit, MRC Centre, Cambridge U.K.

PMID: 2555750
DOI: 10.1016/0304-3940(89)90193-6

Abstract

Using in situ hybridization histochemistry, we have demonstrated that 3 alpha subunit mRNAs of the GABAA receptor are present in different cell populations of the bovine cerebellum. While the alpha 1 mRNA is the most abundant and is present in granule cells, Purkinje cells and stellate/basket cells, the alpha 2 mRNA appears to be confined to the Bergmann glial cell layer. The alpha 3 mRNA is only expressed in the Golgi cells. This differential distribution of GABAA receptor mRNA subtypes suggests a previously unrecognized complexity of GABAergic transmission in the cerebellum.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Cerebellum / cytology
Cerebellum / metabolism*
Nucleic Acid Hybridization
RNA, Messenger / metabolism*
Receptors, GABA-A / metabolism*

Substances

RNA, Messenger
Receptors, GABA-A