Protective role of caffeic acid phenethyl ester on serum cholinesterase inhibition by acute exposure to diazinon in rats

Turk J Med Sci. 2014;44(1):115-20. doi: 10.3906/sag-1210-46.


Aim: To evaluate whether caffeic acid phenethyl ester (CAPE), a flavonoid-like natural compound plentifully found in beeswax, has a protective effect on diazinon-induced serum cholinesterase (ChE) inhibition in rats.

Materials and methods: Animals were divided into 4 groups. The first animal group was not treated with any substance. The second animal group was orally given a 200 mg/kg body weight (bw) sublethal dose of diazinon. The third animal group was injected intraperitoneally with 2.84 mg (10 micromol)/kg bw of CAPE 1 day prior to administration of 200 mg/kg bw of diazinon orally. The fourth animal group was intraperitoneally injected with 2.84 mg (10 tmol)/kg bw of CAPE 30 min after 200 mg/kg bw of diazinon was orally administered.

Results: Analysis of the animal blood samples obtained 48 h after diazinon administration revealed that diazinon decreased serum ChE activity by 75%, while CAPE administration 24 h prior to and 30 min following diazinon application improved serum ChE activity by 25%-32% as compared to levels with diazinon administration only. In silico studies suggest that CAPE prevents diazinon from binding to butyryl ChE due to a higher binding affinity than that of diazinon.

Conclusion: Our laboratory findings suggest that CAPE plays a protective role against butyryl ChE inhibition by diazinon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caffeic Acids / pharmacology*
  • Cholinesterase Inhibitors / toxicity*
  • Cholinesterases / blood*
  • Cholinesterases / chemistry
  • Diazinon / toxicity*
  • Insecticides / toxicity*
  • Male
  • Phenylethyl Alcohol / analogs & derivatives*
  • Phenylethyl Alcohol / pharmacology
  • Rats
  • Rats, Wistar
  • Thermodynamics


  • Caffeic Acids
  • Cholinesterase Inhibitors
  • Insecticides
  • Cholinesterases
  • caffeic acid phenethyl ester
  • Phenylethyl Alcohol
  • Diazinon