In vivo fluorescence microscopy: lessons from observing cell behavior in their native environment

Myunghwan Choi; Sheldon J J Kwok; Seok Hyun Yun

doi:10.1152/physiol.00019.2014

In vivo fluorescence microscopy: lessons from observing cell behavior in their native environment

Physiology (Bethesda). 2015 Jan;30(1):40-9. doi: 10.1152/physiol.00019.2014.

Authors

Myunghwan Choi¹, Sheldon J J Kwok², Seok Hyun Yun³

Affiliations

¹ Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts; and.
² Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts; and Harvard-MIT Health Sciences and Technology, Cambridge, Massachusetts.
³ Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts; and Harvard-MIT Health Sciences and Technology, Cambridge, Massachusetts syun@hms.harvard.edu.

Abstract

Microscopic imaging techniques to visualize cellular behaviors in their natural environment play a pivotal role in biomedical research. Here, we review how recent technical advances in intravital microscopy have enabled unprecedented access to cellular physiology in various organs of mice in normal and diseased states.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review

MeSH terms

Animals
Biomedical Research*
Brain / metabolism
Brain / pathology*
Cell Movement / physiology*
Humans
Microscopy, Fluorescence* / methods
Models, Animal
Skin / pathology

Abstract

Publication types

MeSH terms

Grants and funding