MiR-378 Promotes the Migration of Liver Cancer Cells by Down-Regulating Fus Expression

Cell Physiol Biochem. 2014;34(6):2266-74. doi: 10.1159/000369669. Epub 2014 Dec 9.

Abstract

Background: miR-378 regulates osteoblast differentiation and participates in tumor cell self-renewal and chemo-resistance. However, the function of miR-378 in liver cancer cell migration has not been reported to date.

Methods: miR-378 expression was examined using real-time quantitative PCR. HepG2 cell migration and liver cell invasion were examined using wound-healing and cell invasion assays. Additionally, HepG2 cell metastasis was analyzed in nude mice.

Results: miR-378 over-expression enhances HepG2 cell proliferation, migration and liver cell invasion. Typical metastatic lesions were found in the livers of mice injected with miR-378-transfected cells, and high levels of the CMV promoter were detected in the nodules, indicating that miR-378 promoted the metastasis of the tumor cells to the liver. We also demonstrated that miR-378 down-regulated Fus expression.

Conclusions: Our results suggested that miR-378 enhanced cell migration and metastasis by down-regulating Fus expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carcinoma, Hepatocellular / genetics*
  • Carcinoma, Hepatocellular / pathology
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Gene Expression Regulation, Neoplastic
  • Hep G2 Cells
  • Humans
  • Liver Neoplasms / genetics*
  • Liver Neoplasms / pathology
  • Mice
  • MicroRNAs / genetics*
  • Neoplasm Invasiveness / genetics
  • RNA-Binding Protein FUS / biosynthesis*
  • RNA-Binding Protein FUS / genetics
  • Xenograft Model Antitumor Assays

Substances

  • FUS protein, human
  • MIRN378 microRNA, human
  • MicroRNAs
  • RNA-Binding Protein FUS