Cyclic diguanylic acid and cellulose synthesis in Agrobacterium tumefaciens

J Bacteriol. 1989 Dec;171(12):6649-55. doi: 10.1128/jb.171.12.6649-6655.1989.


The occurrence of the novel regulatory nucleotide bis(3',5')-cyclic diguanylic acid (c-di-GMP) and its relation to cellulose biogenesis in the plant pathogen Agrobacterium tumefaciens was studied. c-di-GMP was detected in acid extracts of 32P-labeled cells grown in various media, and an enzyme responsible for its formation from GTP was found to be present in cell-free preparations. Cellulose synthesis in vivo was quantitatively assessed with [14C]glucose as a tracer. The organism produced cellulose during growth in the absence of plant cells, and this capacity was retained in resting cells. Synthesis of a cellulosic product from UDP-glucose in vitro with membrane preparations was markedly stimulated by c-di-GMP and its precursor GTP and was further enhanced by Ca2+. The calcium effect was attributed to inhibition of a c-di-GMP-degrading enzyme shown to be present in the cellulose synthase-containing membranes.

MeSH terms

  • Arabidopsis Proteins*
  • Cellulose / biosynthesis*
  • Cyclic GMP / analogs & derivatives*
  • Cyclic GMP / metabolism
  • Glucosyltransferases / metabolism
  • Guanosine Triphosphate / metabolism
  • Kinetics
  • Phosphorus Radioisotopes
  • Radioisotope Dilution Technique
  • Rhizobium / growth & development
  • Rhizobium / metabolism*


  • Arabidopsis Proteins
  • Phosphorus Radioisotopes
  • bis(3',5')-cyclic diguanylic acid
  • Guanosine Triphosphate
  • Cellulose
  • Glucosyltransferases
  • PRC1 protein, Arabidopsis
  • cellulose synthase (UDP-forming)
  • Cyclic GMP