A protein binding to the J kappa recombination sequence of immunoglobulin genes contains a sequence related to the integrase motif

Nature. 1989 Dec 21-28;342(6252):934-7. doi: 10.1038/342934a0.


Site-specific recombination requires conserved DNA sequences specific to each system, and system-specific proteins that recognize specific DNA sequences. The site-specific recombinases seem to fall into at least two families, based on their protein structure and chemistry of strand breakage. One of these is the resolvase-invertase family, members of which seem to form a serine-phosphate linkage with DNA. Members of the other family, called the integrase family, contain a conserved tyrosine residue that forms a covalent linkage with the 3'-phosphate of DNA at the site of recombination. Structural comparison of integrases shows that these proteins share a highly conserved 40-residue motif. V-(D)-J recombination of the immunoglobulin gene requires conserved recombination signal sequences (RS) of a heptamer CACTGTG and a T-rich nonamer GGTTTTTGT, which are separated by a spacer sequence of either 12 or 23 bases We have recently purified, almost to homogeneity, a protein that specifically binds to the immunoglobulin J kappa RS containing the 23-base-pair spacer sequence. By synthesizing probes on the basis of partial amino-acid sequences of the purified protein, we have now isolated and characterized the complementary DNA of this protein. The amino-acid sequence deduced from the cDNA sequence reveals that the J kappa RS-binding protein has a sequence similar to the 40-residue motif of integrases of phages, bacteria and yeast, indicating that this protein could be involved in V-(D)-J recombination as a recombinase.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes / immunology
  • Base Sequence
  • Cell Line
  • DNA Nucleotidyltransferases / genetics*
  • DNA Transposable Elements
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / isolation & purification
  • Gene Rearrangement
  • Genes, Immunoglobulin*
  • Immunoglobulin kappa-Chains / genetics*
  • Integrases
  • Mice
  • Molecular Sequence Data
  • Plasmids
  • Recombination, Genetic*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid


  • DNA Transposable Elements
  • DNA-Binding Proteins
  • Immunoglobulin kappa-Chains
  • DNA Nucleotidyltransferases
  • Integrases