A single point mutation confers tetrodotoxin and saxitoxin insensitivity on the sodium channel II

FEBS Lett. 1989 Dec 18;259(1):213-6. doi: 10.1016/0014-5793(89)81531-5.

Abstract

A single point mutation of the rat sodium channel II reduces its sensitivity to tetrodotoxin and saxitoxin by more than three orders of magnitude. The mutation replaces glutamic acid 387 with a glutamine and has only slight effects on the macroscopic current properties, as measured under voltage-clamp in Xenopus oocytes injected with the corresponding cDNA-derived mRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Electric Conductivity
  • Membrane Potentials
  • Mutation
  • Rats
  • Saxitoxin / pharmacology*
  • Sodium Channels / drug effects*
  • Sodium Channels / physiology
  • Sodium Channels / ultrastructure
  • Structure-Activity Relationship
  • Tetrodotoxin / pharmacology*
  • Xenopus laevis

Substances

  • Sodium Channels
  • Saxitoxin
  • Tetrodotoxin