CCR7-dependent trafficking of RORγ⁺ ILCs creates a unique microenvironment within mucosal draining lymph nodes

Nat Commun. 2015 Jan 9;6:5862. doi: 10.1038/ncomms6862.


Presentation of peptide:MHCII by RORγ-expressing group 3 innate lymphoid cells (ILC3s), which are enriched within gut tissue, is required for control of CD4 T-cell responses to commensal bacteria. It is not known whether ILC populations migrate from their mucosal and peripheral sites to local draining secondary lymphoid tissues. Here we demonstrate that ILC3s reside within the interfollicular areas of mucosal draining lymph nodes, forming a distinct microenvironment not observed in peripheral lymph nodes. By photoconverting intestinal cells in Kaede mice we reveal constitutive trafficking of ILCs from the intestine to the draining mesenteric lymph nodes, which specifically for the LTi-like ILC3s was CCR7-dependent. Thus, ILC populations traffic to draining lymph nodes using different mechanisms.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / immunology
  • Cell Movement
  • Female
  • Immunity, Innate
  • Intestinal Mucosa / metabolism
  • Intestines / immunology
  • Light
  • Lymph Nodes / cytology*
  • Lymph Nodes / pathology
  • Lymphocytes / cytology*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microscopy, Fluorescence
  • Mucous Membrane / metabolism*
  • Mucous Membrane / pathology
  • Nuclear Receptor Subfamily 1, Group F, Member 3 / metabolism*
  • Receptors, CCR7 / metabolism*


  • Ccr7 protein, mouse
  • Nuclear Receptor Subfamily 1, Group F, Member 3
  • Receptors, CCR7
  • Rorc protein, mouse