A high-throughput screening assay for identification of inhibitors of the A1AO-ATP synthase of the rumen methanogen Methanobrevibacter ruminantium M1

J Microbiol Methods. 2015 Mar:110:15-7. doi: 10.1016/j.mimet.2014.12.022. Epub 2015 Jan 7.


We report the development of a high-throughput screening platform to identify inhibitors of the membrane-bound A1Ao-ATP synthase from the rumen methanogen Methanobrevibacter ruminantium M1. Inhibitors identified in the screen were tested against growing cultures of M. ruminantium, validating the approach to identify new inhibitors of methanogens.

Keywords: A(1)A(O)-ATP synthase; High-throughput screening; Inhibitor; Methane; Methanobrevibacter ruminantium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / biosynthesis*
  • Adenosine Triphosphate / metabolism
  • Animals
  • Enzyme Assays / methods*
  • High-Throughput Screening Assays*
  • Methanobrevibacter / enzymology*
  • Methanobrevibacter / genetics
  • Methanobrevibacter / growth & development
  • Phylogeny
  • Proton-Translocating ATPases / antagonists & inhibitors*
  • Rumen / microbiology*


  • Adenosine Triphosphate
  • Proton-Translocating ATPases