Determination of dansylated amino acids and biogenic amines in Cannonau and Vermentino wines by HPLC-FLD

Food Chem. 2015 May 15:175:29-35. doi: 10.1016/j.foodchem.2014.11.120. Epub 2014 Nov 27.

Abstract

Free amino acids (AA) and biogenic amines (BA) were quantified for the first time in Cannonau and Vermentino wines, the two most popular "Controlled Designation of Origin" wines from Sardinia (Italy). An analytical method for the simultaneous determination of AA and BA was developed, using selective derivatization with dansyl chloride followed by HPLC with fluorescence detection. Thirty-two compounds were identified in the wines analysed. High levels of AA were found, with proline being the most abundant with average levels of 1244 ± 398 and 1008 ± 281 mg/L in Cannonau and Vermentino wines, respectively. BA were detected at average concentrations <10mg/L, except putrescine which reached 20.5 ± 10.2mg/L in Cannonau wines. Histamine was never detected in any Vermentino wines. γ-Aminobutyric acid, 4-hydroxyproline, glycine, leucine+isoleucine and putrescine proved to be useful for differentiating Cannonau wines from Vermentino wines.

Keywords: Amino acids; Biogenic amines; Dansyl chloride; Fluorescence detection; HPLC; Validation; Wine.

MeSH terms

  • Amino Acids / analysis*
  • Biogenic Amines / analysis*
  • Chromatography, High Pressure Liquid / methods*
  • Dansyl Compounds / analysis*
  • Italy
  • Wine / analysis*

Substances

  • Amino Acids
  • Biogenic Amines
  • Dansyl Compounds
  • dansyl chloride