Analyzing a dipeptide library to identify human dipeptidyl peptidase IV inhibitor

Food Chem. 2015 May 15:175:66-73. doi: 10.1016/j.foodchem.2014.11.131. Epub 2014 Nov 28.

Abstract

Human dipeptidyl peptidase IV (hDPPIV) inhibitors provide an effective strategy for the treatment of type 2 diabetes. Because certain peptides are known to act as hDPPIV inhibitors, a dataset of possible peptides with their inhibition intensities will facilitate the development of functional food for type 2 diabetes. In this study, we examined a total of 337 dipeptides with respect to their hDPPIV inhibitory effects. Amino acid residues at N-termini dominated their inhibition intensities. Particularly highly inhibitory dipeptides discovered included the following novel dipeptides: Thr-His, Asn-His, Val-Leu, Met-Leu, and Met-Met. Using our dataset, prime candidates contributing to the hDPPIV inhibitory effect of soy protein hydrolyzates were successfully identified. Possible dietary proteins potentially able to produce particularly highly hDPPIV inhibitory peptides are also discussed on the basis of the dataset.

Keywords: Dipeptide; Functional food; Human dipeptidyl peptidase IV; Type 2 diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Dietary Proteins / analysis
  • Dietary Proteins / chemistry
  • Dipeptides / analysis
  • Dipeptides / chemistry*
  • Dipeptides / pharmacology*
  • Dipeptidyl-Peptidase IV Inhibitors / analysis
  • Dipeptidyl-Peptidase IV Inhibitors / chemistry*
  • Dipeptidyl-Peptidase IV Inhibitors / pharmacology*
  • Humans
  • Molecular Sequence Data
  • Peptide Library
  • Soybean Proteins / analysis
  • Soybean Proteins / chemistry

Substances

  • Dietary Proteins
  • Dipeptides
  • Dipeptidyl-Peptidase IV Inhibitors
  • Peptide Library
  • Soybean Proteins