Structural characterization of O- and C-glycosylating variants of the landomycin glycosyltransferase LanGT2

Abstract

The structures of the O-glycosyltransferase LanGT2 and the engineered, C-C bond-forming variant LanGT2S8Ac show how the replacement of a single loop can change the functionality of the enzyme. Crystal structures of the enzymes in complex with a nonhydrolyzable nucleotide-sugar analogue revealed that there is a conformational transition to create the binding sites for the aglycon substrate. This induced-fit transition was explored by molecular docking experiments with various aglycon substrates.

Keywords: C-glycosylation; Friedel-Crafts alkylation; carbasugars; enzyme engineering; glycosyltransferases.