Petroselinum crispum has antioxidant properties, protects against DNA damage and inhibits proliferation and migration of cancer cells

J Sci Food Agric. 2015 Oct;95(13):2763-71. doi: 10.1002/jsfa.7078. Epub 2015 Feb 19.

Abstract

Background: Petroselinum crispum (English parsley) is a common herb of the Apiaceae family that is cultivated throughout the world and is widely used as a seasoning condiment. Studies have shown its potential as a medicinal herb. In this study, P. crispum leaf and stem extracts were evaluated for their antioxidant properties, protection against DNA damage in normal 3T3-L1 cells, and the inhibition of proliferation and migration of the MCF-7 cells.

Results: The dichloromethane extract of P. crispum exhibited the highest phenolic content (42.31 ± 0.50 mg GAE g(-1) ) and ferric reducing ability (0.360 ± 0.009 mmol g(-1) ) of the various extractions performed. The extract showed DPPH radical scavenging activity with an IC50 value of 3310.0 ± 80.5 µg mL(-1) . Mouse fibroblasts (3T3-L1) pre-treated with 400 µg mL(-1) of the extract showed 50.9% protection against H2 O2 -induced DNA damage, suggesting its potential in cancer prevention. The extract (300 µg mL(-1) ) inhibited H2 O2 -induced MCF-7 cell migration by 41% ± 4%. As cell migration is necessary for metastasis of cancer cells, inhibition of migration is an indication of protection against metastasis.

Conclusion: Petroselinum crispum has health-promoting properties with the potential to prevent oxidative stress-related diseases and can be developed into functional food.

Keywords: DNA protection; MCF-7 cell migration; Petroselinum crispum; antioxidant activity; antiproliferative activity; hydrogen peroxide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Animals
  • Antineoplastic Agents, Phytogenic / pharmacology
  • Antineoplastic Agents, Phytogenic / therapeutic use
  • Antioxidants / pharmacology*
  • Biphenyl Compounds / metabolism
  • Breast Neoplasms* / metabolism
  • Breast Neoplasms* / prevention & control
  • Cell Movement / drug effects*
  • Cell Proliferation / drug effects*
  • DNA / drug effects
  • DNA Damage*
  • Female
  • Functional Food
  • Humans
  • Hydrogen Peroxide / metabolism
  • MCF-7 Cells
  • Mice
  • Neoplasm Metastasis / prevention & control
  • Oxidative Stress / drug effects
  • Petroselinum / chemistry*
  • Phenols / analysis
  • Phenols / pharmacology*
  • Phenols / therapeutic use
  • Phytotherapy
  • Picrates / metabolism
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Plant Leaves

Substances

  • Antineoplastic Agents, Phytogenic
  • Antioxidants
  • Biphenyl Compounds
  • Phenols
  • Picrates
  • Plant Extracts
  • DNA
  • Hydrogen Peroxide
  • 1,1-diphenyl-2-picrylhydrazyl