[Blood sampling using "dried blood spot": a clinical biology revolution underway?]

Ann Biol Clin (Paris). 2015 Jan-Feb;73(1):25-37. doi: 10.1684/abc.2014.1007.
[Article in French]


Blood testing using the dried blood spot (DBS) is used since the 1960s in clinical analysis, mainly within the framework of the neonatal screening (Guthrie test). Since then numerous analytes such as nucleic acids, small molecules or lipids, were successfully measured on the DBS. While this pre-analytical method represents an interesting alternative to classic blood sampling, its use in routine is still limited. We review here the different clinical applications of the blood sampling on DBS and estimate its future place, supported by the new methods of analysis as the LC-MS mass spectrometry.

Keywords: ELISA; PCR; dried blood spot; mass spectrometry; multiplex; pre-analytical.

Publication types

  • English Abstract
  • Review

MeSH terms

  • Blood Proteins / analysis
  • Blood Specimen Collection / methods*
  • Blood Specimen Collection / trends*
  • Carbohydrates / analysis
  • Carbohydrates / blood
  • Dried Blood Spot Testing* / methods
  • Dried Blood Spot Testing* / trends
  • Genomics / methods
  • Humans
  • Lipids / analysis
  • Lipids / blood
  • Nucleic Acids / analysis
  • Nucleic Acids / isolation & purification
  • Peptides / analysis
  • Peptides / blood
  • Xenobiotics / analysis
  • Xenobiotics / blood


  • Blood Proteins
  • Carbohydrates
  • Lipids
  • Nucleic Acids
  • Peptides
  • Xenobiotics