Comparison of various methods used to detect biofilm production of Candida species

doi:10.7860/JCDR/2014/10445.5147

. 2014 Nov;8(11):DC18-c20.

doi: 10.7860/JCDR/2014/10445.5147. Epub 2014 Nov 20.

Comparison of various methods used to detect biofilm production of Candida species

Rahul P Dhale¹, M V Ghorpade², C A Dharmadhikari³

Affiliations

¹ Student, Department of Microbiology, Krishna Institute of Medical Sciences Deemed University , Karad, District Satara, Maharashtra, India .
² Professor, Department of Microbiology, Krishna Institute of Medical Sciences Deemed University , Karad, District Satara, Maharashtra, India .
³ Professor, Department of Microbiology, Tatyasaheb Kore Dental College and Research Centre , New Pargaon, District Kolhapur, Maharashtra, India .

PMID: 25584219
PMCID: PMC4290237
DOI: 10.7860/JCDR/2014/10445.5147

Comparison of various methods used to detect biofilm production of Candida species

Rahul P Dhale et al. J Clin Diagn Res. 2014 Nov.

. 2014 Nov;8(11):DC18-c20.

doi: 10.7860/JCDR/2014/10445.5147. Epub 2014 Nov 20.

Authors

Rahul P Dhale¹, M V Ghorpade², C A Dharmadhikari³

Affiliations

¹ Student, Department of Microbiology, Krishna Institute of Medical Sciences Deemed University , Karad, District Satara, Maharashtra, India .
² Professor, Department of Microbiology, Krishna Institute of Medical Sciences Deemed University , Karad, District Satara, Maharashtra, India .
³ Professor, Department of Microbiology, Tatyasaheb Kore Dental College and Research Centre , New Pargaon, District Kolhapur, Maharashtra, India .

PMID: 25584219
PMCID: PMC4290237
DOI: 10.7860/JCDR/2014/10445.5147

Abstract

Purpose: The biofilm of organisms can be considered as a virulence factor because of the resistance towards antimicrobial agents. Formation of Candida biofilms are observed in blood, mucosal surface and most medical devices i.e. nonliving objects in patient's body. The study was undertaken to conclude the most sensitive and specific test for detection of biofilm formation.

Materials and methods: Quantitative measurement of biofilm formation was assessed by three methods- 1) XTT reductionassay, 2) Percentage Transmission (%T) and 3) Crystal Violet assay in microtitre plate for 425 Candida isolates.

Results: Out of 425 Candida strains, XTT reduction assay gave 72 Biofilm positive strains accounting for 16.94 %. The percentage transmission (% T) method gave 56 strains Biofilm positive (13.17 %) and Crystal violet assay gave 50 positive strains (11.76 %). Compared to Percentage Transmission (% T) and Crystal violet method, XTT reduction assay gave maximum percentage of Biofilm positivity.

Conclusion: In comparison of 3 methods used for detection of biofilm formation, XTT reduction assay was the most sensitive and specific method.

Keywords: Biofilm; XTT reduction assay.

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References

1. Kumar A, Prasad R. Biofilms. Jk Science. 2006;8(1):14–17.
1. Biofilms- The new microbial order, [homepage on the internet] Available from: medicalmycology.org/Biofilms.htm.
1. Taff HT, Nett JE, Andes DR. Comparative analysis of Candida biofilm quantitation assays. Medical Mycology. Online. 2011:1–5. - PMC - PubMed
1. Shin JH, Kee SJ, Shin MJ, Kim S H, Shin DH, Lee SK, et al. Biofilm Production by Isolates of Candida Species Recovered from Nonneutropenic Patients: Comparison of Bloodstream Isolates with Isolates from Other Sources. J Clin Microbiol. 2002;40(4):1244–48. - PMC - PubMed
1. Cruickshank’s Medical Microbiology. 12th Edition. Churchill Livingstone; Apr 1975. (PBS- page 92)

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[1] Kumar A, Prasad R. Biofilms. Jk Science. 2006;8(1):14–17.

[2] Kumar A, Prasad R. Biofilms. Jk Science. 2006;8(1):14–17.

[3] Biofilms- The new microbial order, [homepage on the internet] Available from: medicalmycology.org/Biofilms.htm.

[4] Biofilms- The new microbial order, [homepage on the internet] Available from: medicalmycology.org/Biofilms.htm.

[5] Taff HT, Nett JE, Andes DR. Comparative analysis of Candida biofilm quantitation assays. Medical Mycology. Online. 2011:1–5. - PMC - PubMed

[6] Taff HT, Nett JE, Andes DR. Comparative analysis of Candida biofilm quantitation assays. Medical Mycology. Online. 2011:1–5. - PMC - PubMed

[7] Shin JH, Kee SJ, Shin MJ, Kim S H, Shin DH, Lee SK, et al. Biofilm Production by Isolates of Candida Species Recovered from Nonneutropenic Patients: Comparison of Bloodstream Isolates with Isolates from Other Sources. J Clin Microbiol. 2002;40(4):1244–48. - PMC - PubMed

[8] Shin JH, Kee SJ, Shin MJ, Kim S H, Shin DH, Lee SK, et al. Biofilm Production by Isolates of Candida Species Recovered from Nonneutropenic Patients: Comparison of Bloodstream Isolates with Isolates from Other Sources. J Clin Microbiol. 2002;40(4):1244–48. - PMC - PubMed

[9] Cruickshank’s Medical Microbiology. 12th Edition. Churchill Livingstone; Apr 1975. (PBS- page 92)

[10] Cruickshank’s Medical Microbiology. 12th Edition. Churchill Livingstone; Apr 1975. (PBS- page 92)

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Comparison of various methods used to detect biofilm production of Candida species

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Comparison of various methods used to detect biofilm production of Candida species

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