Abstract
We have investigated the effects of recombinant interleukin-1 beta (rIL-1 beta) on phospholipase A2 activity (PLA2), PLA2 messenger RNA levels, and eicosanoid production in rabbit chondrocytes. Phospholipase A2 activity increased 5 fold with exposure to 1.6 x 10(-11) M rIL-1 beta for 20 hours. An mRNA specific for an extracellular PLA2 was detected by RNA blot hybridization after treatment of the cells with rIL-1 beta. Hydroxylated derivatives of arachidonic acid, including prostaglandins, leukotriene B4, 5-hydroxyeicosa 6E, 8Z, 11Z, 14Z-tetraenoic acid, 12-hydroxyeicosa 5Z, 8Z, 10E, 14Z-tetraenoic acid and 15-hydroxyeicosa 5Z, 8Z, 11Z, 13E-tetraenoic acid increased in cells after rIL-1 beta treatment.
MeSH terms
-
Animals
-
Arachidonic Acids / metabolism
-
Cartilage, Articular / enzymology*
-
DNA Probes
-
Dinoprost / biosynthesis
-
Dinoprostone / biosynthesis
-
Eicosanoids / biosynthesis*
-
Hydroxyeicosatetraenoic Acids / biosynthesis
-
Interleukin-1 / pharmacology*
-
Kinetics
-
Leukotriene B4 / biosynthesis
-
Male
-
Nucleic Acid Hybridization
-
Phospholipases / metabolism*
-
Phospholipases A / genetics
-
Phospholipases A / metabolism*
-
Phospholipases A2
-
Prostaglandin D2 / biosynthesis
-
RNA, Messenger / analysis*
-
Rabbits
-
Recombinant Proteins
Substances
-
Arachidonic Acids
-
DNA Probes
-
Eicosanoids
-
Hydroxyeicosatetraenoic Acids
-
Interleukin-1
-
RNA, Messenger
-
Recombinant Proteins
-
Leukotriene B4
-
Dinoprost
-
Phospholipases
-
Phospholipases A
-
Phospholipases A2
-
Dinoprostone
-
Prostaglandin D2