A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants

Nat Commun. 2015 Jan 16;6:6043. doi: 10.1038/ncomms7043.

Abstract

Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification of dynamic changes in JA distribution in response to stress with high spatiotemporal sensitivity. We show that Jas9-VENUS abundance is dependent on bioactive JA isoforms, the COI1 co-receptor, a functional Jas motif and proteasome activity. We demonstrate the utility of Jas9-VENUS to analyse responses to JA in planta at a cellular scale, both quantitatively and dynamically. This included using Jas9-VENUS to determine the cotyledon-to-root JA signal velocities on wounding, revealing two distinct phases of JA activity in the root. Our results demonstrate the value of developing quantitative sensors such as Jas9-VENUS to provide high-resolution spatiotemporal data about hormone distribution in response to plant abiotic and biotic stresses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / methods*
  • Cotyledon / metabolism
  • Cyclopentanes / analysis*
  • Cyclopentanes / metabolism*
  • Oxylipins / analysis*
  • Oxylipins / metabolism*
  • Plant Roots / metabolism
  • Plants / metabolism*

Substances

  • Cyclopentanes
  • Oxylipins
  • jasmonic acid