Redox sensor proteins for highly sensitive direct imaging of intracellular redox state

Biochem Biophys Res Commun. 2015 Feb 13;457(3):242-8. doi: 10.1016/j.bbrc.2014.12.095. Epub 2015 Jan 13.


Intracellular redox state is a critical factor for fundamental cellular functions, including regulation of the activities of various metabolic enzymes as well as ROS production and elimination. Genetically-encoded fluorescent redox sensors, such as roGFP (Hanson, G. T., et al. (2004)) and Redoxfluor (Yano, T., et al. (2010)), have been developed to investigate the redox state of living cells. However, these sensors are not useful in cells that contain, for example, other colored pigments. We therefore intended to obtain simpler redox sensor proteins, and have developed oxidation-sensitive fluorescent proteins called Oba-Q (oxidation balance sensed quenching) proteins. Our sensor proteins derived from CFP and Sirius can be used to monitor the intracellular redox state as their fluorescence is drastically quenched upon oxidation. These blue-shifted spectra of the Oba-Q proteins enable us to monitor various redox states in conjunction with other sensor proteins.

Keywords: Biosensor; Disulfide; Fluorescence; Oxidation-reduction; Redox regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / methods
  • Glutathione / metabolism
  • Glutathione Disulfide / metabolism
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism*
  • HeLa Cells
  • Humans
  • Intracellular Fluid / metabolism*
  • Mutagenesis
  • Oxidation-Reduction
  • Quantum Theory
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Spectrometry, Fluorescence


  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • Glutathione
  • Glutathione Disulfide