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, 33 (4), 208-15

Ethanolic Extract of Aloe Vera Ameliorates Sciatic Nerve Ligation Induced Neuropathic Pain

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Ethanolic Extract of Aloe Vera Ameliorates Sciatic Nerve Ligation Induced Neuropathic Pain

Swetha Kanyadhara et al. Anc Sci Life.

Abstract

Background: Aloe vera is being used since ages by human kind for treating various ailments including various inflammatory conditions, but scientific validation has not been done for analgesic activity against neuropathic pain.

Objective: The current study was designed to systematically evaluate the therapeutic potential of the ethanolic extract of A. vera (EEAV) against sciatic nerve ligation (SCNL) induced neuropathic pain.

Materials and methods: Nociceptive threshold of EEAV against thermal hyperalgesia, chemical hyperalgesia and mechanical allodynia were performed on 0, 7, 14 and 21(st) day post-SCNL. Serum total protein, serum nitrite, in vivo anti-oxidant parameters and lipid peroxidation (LPO) were estimated. Sciatic nerve homogenate was used to estimate myeloperoxidase (MPO) and calcium levels. Histopathology of the sciatic nerve was done to confirm the biochemical findings.

Results: Treatment with ethanolic extract has increased the threshold for the nociception in thermal hyperalgesia, chemical hyperalgesia and mechanical allodynia models. A significant improvement of in vivo anti-oxidant parameters and decreased LPO levels were observed on treatment with A. vera. Significant decrease in serum nitrite, protein, calcium and MPO levels were observed, indicating protection against damage caused by SCNL.

Conclusion: The results of the present study validate the use of EEAV to treat neuropathic pain. This effect may be attributed to the decreased migration of neutrophils and due to the anti-oxidant properties of A. vera. Further studies to confirm the mechanism of action will help develop suitable A. vera formulations for neuropathic pain therapy.

Keywords: Aloe vera; chronic constriction injury; neuropathic pain; sciatic nerve ligation.

Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Effect of ethanolic extract of Aloe vera on serum protein and serum nitrite. Units: Serum protein – g/dL; serum nitrite – μg/mL; all values are shown as mean ± standard error of the mean and n = 8; *indicates P < 0.05 when compared with normal; **indicates P < 0.05 when compared with control group
Figure 2
Figure 2
Effect of ethanolic extract of Aloe vera on calcium and myeloperoxidase. Units of calcium (ppm/mg); myeloperoxidase (U/g); all values are shown as mean ± standard error of the mean and n = 8; *indicates P < 0.05 when compared with normal; **indicates P < 0.05 when compared with control group
Figure 3
Figure 3
Effect of ethanolic extract of Aloe vera on super oxide dismutase (SOD), catalase and glutathione (GSH). Units of SOD: U/g; catalase: H2O2 consumed/min/mg of tissue; GSH: μg of GSH/g of tissue; all values are shown as mean ± standard error of the mean and n = 8; *indicates P < 0.05 when compared with normal; **indicates P < 0.05 when compared with control group
Figure 4
Figure 4
Effect of ethanolic extract of Aloe vera on lipid peroxidation (LPO). Units of LPO (nmoles of malondialdehyde/mg of tissue); all values are shown as mean ± standard error of the mean and n = 8; *indicates P < 0.05 when compared with normal; **indicates P < 0.05 when compared with control group
Figure 5
Figure 5
Photo micrographs of the sciatic nerve stained with toulidine blue (×40). (a) Group I (Normal) = Treated with vehicle; (b) Group II (Control) = Sciatic nerve ligation (SCNL); (c) Group III (Standard) = SCNL + Gabapentin (9 mg/kg, p.o.); (d) Group IV (Low dose) = SCNL + Aloe vera extract (100 mg/kg, p.o.); (e) Group V (High dose) = SCNL + A. vera extract (300 mg/kg, p.o.)

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