Switch regulation of interleukin-1 beta in downstream of inflammatory cytokines induced by two micro-sized silica particles on differentiated THP-1 macrophages

Ting Zhou; Xiuqing Cui; Yun Zhou; Jiali Guo; Yi Rong; Weihong Chen

doi:10.1016/j.etap.2014.12.017

Switch regulation of interleukin-1 beta in downstream of inflammatory cytokines induced by two micro-sized silica particles on differentiated THP-1 macrophages

Environ Toxicol Pharmacol. 2015 Jan;39(1):457-66. doi: 10.1016/j.etap.2014.12.017. Epub 2015 Jan 3.

Authors

Ting Zhou¹, Xiuqing Cui², Yun Zhou², Jiali Guo², Yi Rong², Weihong Chen³

Affiliations

¹ Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; School of Public Health, Wuhan University of Science and Technology, Wuhan, Hubei, 430065, China.
² Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
³ Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. Electronic address: wchen@mails.tjmu.edu.cn.

PMID: 25596480
DOI: 10.1016/j.etap.2014.12.017

Abstract

To investigate the regulated role of IL-1β in initiating and maintaining inflammation, PMA-differentiated THP-1 macrophages were exposed to two micro-sized crystalline silica particles (Si3-5μm and Si1μm) from 3h to 24h, respectively. Cytotoxicity and inflammatory cytokines (IL-1β, TNF-α and IL-6) expressions measured showed that they were induced by both silica particles in positive dose-dependent manners. The levels of inflammatory cytokines induced by Si1μm were higher than those induced by Si3-5μm at low concentration. When pretreated with anti-human IL-1β, not only the high levels of IL-1β but also elevated TNF-α and IL-6 induced by both silica particles were remarkably blocked, especially Si1μm particle. In addition, recombinant human IL-1β protein could induce macrophages to strikingly augment TNF-α and IL-6 expressions. Our data suggest that IL-1β could play a critical role of switching regulation in the downstream inflammation induced by micro-sized silica particles.

Keywords: IL-1β; Inflammatory cytokines; Macrophages; Silica particles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies / pharmacology
Cell Line, Tumor
Cell Survival / drug effects
Cytokines / metabolism*
Humans
Macrophages / drug effects*
Macrophages / metabolism
Particle Size
Silicon Dioxide / toxicity*

Substances

Antibodies
Cytokines
Silicon Dioxide