Effect of 1-year dietary supplementation with vitaminized olive oil on markers of bone turnover and oxidative stress in healthy post-menopausal women

Endocrine. 2015 Nov;50(2):326-34. doi: 10.1007/s12020-015-0529-6. Epub 2015 Jan 20.


Osteoporosis represents a serious health problem worldwide associated with an increased risk of fractures and mortality. Nutrition should form part of bone disease prevention strategies, especially in the light of the population ageing and the diet effect on bone health. Thus the study aimed at verifying whether 1 year of oral supplementation with either extra virgin olive oil (VOO) enriched with vitamins D3, K1 and B6 (VitVOO) or VOO used as placebo (PlaVOO) is able to modify some bone turnover and oxidative stress markers. Bone mineral density (BMD) was assessed in 60 healthy post-menopausal women together with the bone vitamin K status by measuring undercarboxylated osteocalcine (ucOC) plasma levels, the ratio between ucOC and carboxylated osteocalcine (UCR) and the relations with oxidative stress markers. After 1 year (T 1), subjects taking VitVOO showed lower ucOC levels than those taking PlaVOO; the same trend was found for UCR. As far as BMD is concerned, a significant increase in T-score at T 1 in VitVOO subjects compared to PlaVOO was found. All oxidative stress markers as thiobarbituric acid reactive substances, lipid hydroperoxides and conjugated dienes showed a significant reduction after VitVOO supplementation, whilst plasma total antioxidant capacity values was significantly increased in VitVOO group compared to PlaVOO group at T 1. It might be suggested that the use of VitVOO in the diet of post-menopausal women could represent a proper tool for bone protection and a useful strategy against oxidative stress and related diseases, thus confirming the antioxidant role played by the added vitamins.

Keywords: Bone mineral density; Osteocalcin; Osteoporosis; Oxidative stress; Vitaminized oil.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Density / drug effects*
  • Cholecalciferol / administration & dosage
  • Cholecalciferol / pharmacology
  • Dietary Supplements
  • Female
  • Humans
  • Middle Aged
  • Olive Oil / administration & dosage
  • Olive Oil / pharmacology*
  • Outcome Assessment, Health Care*
  • Oxidative Stress / drug effects*
  • Postmenopause / blood*
  • Postmenopause / drug effects*
  • Vitamin B 6 / administration & dosage
  • Vitamin B 6 / pharmacology
  • Vitamin K 1 / administration & dosage
  • Vitamin K 1 / pharmacology
  • Vitamins / administration & dosage
  • Vitamins / pharmacology*


  • Olive Oil
  • Vitamins
  • Cholecalciferol
  • Vitamin B 6
  • Vitamin K 1