Preventive effect of Pueraria mirifica on testosterone-induced prostatic hyperplasia in Sprague Dawley rats

Andrologia. 2015 Dec;47(10):1153-9. doi: 10.1111/and.12396. Epub 2015 Jan 20.


Pueraria mirifica (PM) extract contains phytoestrogen daidzein and genistein. In this study, we investigated the protective effect of PM extract, daidzein and genistein on a testosterone-induced prostatic hyperplasia in rats. Testosterone was administered at 3 mg kg(-1) to rats followed by the PM extract, daidzein and genistein for a period of 30 days with finasteride as positive control. The testosterone level was increased, indicating inhibition of 5α-reductase converting testosterone to dihydrotestosterone. This was confirmed by prostate-specific antigen level that significantly decreased when treated with PM extract, daidzein and genistein. The PM extract, daidzein and genistein reduced the increase in the prostate/body weight ratio in testosterone-induced rats. This gives indication that PM extract, daidzein and genistein possessed protective activity for the treatment of benign prostatic hyperplasia. The analysis of histoarchitechture of the prostate has also shown that there was a significant improvement in prostatic cells of the testosterone-induced rats when treated with PM extract, daidzein and genistein.

Keywords: Benign prostatic hyperplasia; Pueraria mirifica; daidzein; genistein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Weight / drug effects
  • Genistein / pharmacology
  • Isoflavones / pharmacology
  • Male
  • Organ Size / drug effects
  • Phytotherapy / methods*
  • Plant Extracts / pharmacology*
  • Plant Roots
  • Prostate / drug effects
  • Prostatic Hyperplasia / chemically induced
  • Prostatic Hyperplasia / prevention & control*
  • Pueraria*
  • Rats
  • Rats, Sprague-Dawley
  • Testosterone / blood
  • Testosterone / pharmacology*


  • Isoflavones
  • Plant Extracts
  • Testosterone
  • daidzein
  • Genistein