Gold nanoparticles coated zinc oxide nanorods as the matrix for enhanced L-lactate sensing

Yanguang Zhao; Xiaofei Fang; Yousong Gu; Xiaoqin Yan; Zhuo Kang; Xin Zheng; Pei Lin; Leichao Zhao; Yue Zhang

doi:10.1016/j.colsurfb.2014.12.053

Gold nanoparticles coated zinc oxide nanorods as the matrix for enhanced L-lactate sensing

Colloids Surf B Biointerfaces. 2015 Feb 1:126:476-80. doi: 10.1016/j.colsurfb.2014.12.053. Epub 2015 Jan 7.

Authors

Yanguang Zhao¹, Xiaofei Fang¹, Yousong Gu¹, Xiaoqin Yan², Zhuo Kang¹, Xin Zheng¹, Pei Lin¹, Leichao Zhao¹, Yue Zhang³

Affiliations

¹ State Key Laboratory for Advanced Metals and Materials, School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, People's Republic of China.
² State Key Laboratory for Advanced Metals and Materials, School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, People's Republic of China. Electronic address: xqyan@mater.ustb.edu.cn.
³ State Key Laboratory for Advanced Metals and Materials, School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, People's Republic of China; Key Laboratory of New Energy Materials and Technologies, University of Science and Technology Beijing, Beijing 100083, People's Republic of China. Electronic address: yuezhang@ustb.edu.cn.

PMID: 25601093
DOI: 10.1016/j.colsurfb.2014.12.053

Abstract

In this study, an enzymatic electrochemical biosensor for L-lactate detection was proposed. The device was developed based on gold nanoparticles (Au NPs) modified zinc oxide nanorods (ZnO NRs). The sensing performance of the device was examined by cyclic voltammetry and amperometry. Compared with pristine ZnO based biosensor, Au/ZnO based sensor exhibited higher sensitivity of 24.56 μA cm(-2) mM(-1), smaller K(M)(app) of 1.58 mM, lower detection limit of 6 μM and wider linear range of 10 μM-0.6 mM for L-lactate detection. The introduction of Au NPs enhances electro-catalytic ability and electron migration, which contributes to the improvement of the sensing performance. Hence, the results confirm the essential character of Au NPs in such semiconductor based electrochemical biosensing system.

Keywords: Electrochemical biosensor; Gold nanoparticles; Lactate oxidase; Zinc oxide; l-Lactate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques
Electrochemical Techniques
Gold / chemistry*
Lactic Acid / analysis*
Metal Nanoparticles / chemistry*
Nanotubes / chemistry*
Zinc Oxide / chemistry*

Substances

Lactic Acid
Gold
Zinc Oxide