A new approach for optical assessment of directional anisotropy in turbid media

J Biophotonics. 2016 Jan;9(1-2):100-8. doi: 10.1002/jbio.201400124. Epub 2015 Jan 19.


A study of polarized light transport in scattering media exhibiting directional anisotropy or linear birefringence is presented in this paper. Novel theoretical and experimental methodologies for the quantification of birefringent alignment based on out-of-plane polarized light transport are presented here. A polarized Monte Carlo model and a polarimetric imaging system were devised to predict and measure the impact of birefringence on an impinging linearly polarized light beam. Ex-vivo experiments conducted on bovine tendon, a biological sample consisting of highly packed type I collagen fibers with birefringent property, showed good agreement with the analytical results.

Keywords: Monte Carlo; birefringence; directional anisotropy; out-of-plane; polarized light imaging.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Anisotropy
  • Birefringence
  • Cattle
  • Collagen / metabolism
  • Light*
  • Monte Carlo Method*
  • Optical Imaging*
  • Scattering, Radiation*
  • Tendons / metabolism


  • Collagen