Specific identification of Bordetella pertussis by the polymerase chain reaction

Res Microbiol. 1989 Sep;140(7):477-87. doi: 10.1016/0923-2508(89)90069-7.


Oligonucleotide primers were used to amplify specific DNA regions of the Bordetella pertussis genome by the polymerase chain reaction. One pair of primers, PTp1/PTp2, identified a 191-bp DNA fragment located in the regulatory region of the pertussis toxin operon; a second pair of primers led to amplification of a 121-bp DNA piece located in an insertion-like element specific to B. pertussis. Both sets of primers were able to discriminate between the pathogen and related Bordetella species; they detected down to 6 bacteria and appeared suitable for routine detection of B. pertussis in clinical specimens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bordetella pertussis / isolation & purification*
  • DNA
  • Gene Amplification*
  • Genomic Library*
  • Humans
  • Oligonucleotides
  • Polymerase Chain Reaction*
  • Whooping Cough / diagnosis
  • Whooping Cough / microbiology*


  • Oligonucleotides
  • DNA