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, 41 (5), 281-6

Impact of Hydration Media on Ex Vivo Corneal Elasticity Measurements

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Impact of Hydration Media on Ex Vivo Corneal Elasticity Measurements

Janice Dias et al. Eye Contact Lens.

Abstract

Objectives: To determine the effect of hydration media on ex vivo corneal elasticity.

Methods: Experiments were conducted on 40 porcine eyes retrieved from an abattoir (10 eyes each for phosphate-buffered saline (PBS), balanced salt solution, Optisol, 15% dextran). The epithelium was removed, and the cornea was excised with an intact scleral rim and placed in 20% dextran overnight to restore its physiological thickness. For each hydration media, corneas were evenly divided into two groups: one with an intact scleral rim and the other without. Corneas were mounted onto a custom chamber and immersed in a hydration medium for elasticity testing. Although in each medium, corneal elasticity measurements were performed for 2 hr: at 5-min intervals for the first 30 min and then 15-min intervals for the remaining 90 min. Elasticity testing was performed using nanoindentation with spherical indenters, and Young modulus was calculated using the Hertz model. Thickness measurements were taken before and after elasticity testing.

Results: The percentage change in corneal thickness and elasticity was calculated for each hydration media group. Balanced salt solution, PBS, and Optisol showed an increase in thickness and Young moduli for corneas with and without an intact scleral rim. Fifteen percent dextran exhibited a dehydrating effect on corneal thickness and provided stable maintenance of corneal elasticity for both groups.

Conclusions: Hydration media affects the stability of corneal thickness and elasticity measurements over time. Fifteen percent dextran was most effective in maintaining corneal hydration and elasticity, followed by Optisol.

Conflict of interest statement

The authors do not have any proprietary or financial interest in any of the devices presented.

Figures

Figure 1
Figure 1
Bar Graph of the Average Corneal Thickness Percentage Change. A bar graph comparing the thickness percentage changes after 120 minutes in different hydration media. Samples with the intact sclera had a greater change in thickness than samples without the intact sclera.
Figure 2
Figure 2
Bar Graph of the Average Corneal Elasticity Percentage Change. A bar graph comparing the corneal Young’s modulus percentage changes after 120 minutes in different hydration media. Samples with the intact sclera had a greater change in Young’s modulus than samples without the intact sclera.
Figure 3
Figure 3
Scatter Plots of the Corneal Elasticity Profile during AFM Testing for each Hydration Medium. For one representative sample per hydration media, Young’s modulus is plotted as a function of time. Corneal samples with and without intact scleral rim are included in each graph: A.) 15% Dextran, B.) BSS, C.) PBS, and D.) Optisol.

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