Associations of genetics and sampling time with levels of interleukin-2 activity

Vet Immunol Immunopathol. 1989 Nov 15;22(4):333-43. doi: 10.1016/0165-2427(89)90169-4.

Abstract

The interleukin-2 (IL-2) assay has become a useful tool for examining the cellular immune response. Because of a lack of avian IL-2-dependent cell lines, the avian IL-2 assay in its present state, however, is not currently as powerful as its mammalian counterpart in effectively evaluating levels of IL-2 activity. The use of an avian IL-2 reference standard, Percoll-enriched populations of lymphoblasts, and sample collection times were examined to optimize the assay for comparing levels of IL-2 activity in a large number of birds. The reference standard was effective in accounting for assay-to-assay variance. Percoll-enriched populations of responding lymphoblasts were more sensitive to IL-2 than nonseparated populations of cultured peripheral blood lymphocytes. Levels of IL-2 activity of cells isolated from the same bird did not change within a 1-week period, although differences did approach significance. In addition, levels of IL-2 activity differed between genetically distinct populations.

MeSH terms

  • Animals
  • Cell Line
  • Cell Separation
  • Chickens / genetics
  • Chickens / immunology*
  • Concanavalin A / pharmacology
  • Female
  • Genotype
  • Interleukin-2 / biosynthesis*
  • Interleukin-2 / genetics
  • Lymphocytes / metabolism*
  • Male
  • Mathematics
  • Povidone
  • Reference Standards
  • Silicon Dioxide
  • Time Factors

Substances

  • Interleukin-2
  • Concanavalin A
  • Percoll
  • Silicon Dioxide
  • Povidone