Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase

J Integr Med. 2015 Jan;13(1):25-33. doi: 10.1016/S2095-4964(15)60157-1.

Abstract

Objective: The roots of Chlorophytum borivilanum are used in traditional medicine for the treatment of arthritis and inflammation. The aim of the work was to evaluate the anti-inflammatory activity of isolated saponins from Chlorophytum borivilianum (ISCB).

Methods: The ISCB was screened using the carrageenan-induced paw edema, histamine-induced paw edema, cotton pellet-induced granuloma, and Freund's adjuvant-induced arthritis in rats at orally administered doses of 3, 10, and 30 mg/kg. Effect of ISCB on histone deacetylase (HDAC) level was measured by the HDAC assay at the highest dose (30 mg/kg).

Results: The results showed that the ISCB significantly reduced carrageenan-induced inflammation, histamine-induced inflammation, cotton pellet-induced granuloma and Freund's adjuvant-induced arthritis in rats. The ISCB at a dose of 30 mg/kg significantly inhibited HDAC level in rat paw tissue.

Conclusion: It is concluded that saponins isolated from roots of C. borivilianum possess anti-inflammatory and anti-arthritic properties. ISCB may act by inhibiting histamine, prostaglandin and HDAC. This suggests that ISCBs have potential for therapeutic use in the treatment of inflammation and arthritis.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Arthritis, Experimental / drug therapy*
  • Female
  • Histone Deacetylase Inhibitors / pharmacology*
  • Histone Deacetylases / metabolism
  • Liliaceae / chemistry*
  • Male
  • Plant Roots / chemistry
  • Rats
  • Rats, Wistar
  • Saponins / pharmacology*
  • Saponins / therapeutic use
  • Saponins / toxicity

Substances

  • Anti-Inflammatory Agents
  • Histone Deacetylase Inhibitors
  • Saponins
  • Histone Deacetylases