Abstract
Specific B-cell subsets can regulate T-cell immune responses, and are termed regulatory B cells (Breg). The majority of Breg cells described in mouse and man have been identified by IL-10 production and are known to suppress allergy and autoimmunity. However, Breg cell mediated immune suppression, independent of IL-10, also occurs. Here we show that Breg cells play a critical role in regulating humoral immunity mediated by CD4(+)CXCR5(+)PD-1(+) follicular helper T cells, and can suppress inflammation in autoimmune disease through elevated expression of PD-L1. We have also identified that these B cells are resistant to αCD20 B-cell depletion. This work describes how Breg cells are critical in humoral homoeostasis and may have implications for the regulation of autoimmune diseases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Animals
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Antibodies, Monoclonal / chemistry
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Autoimmunity
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B-Lymphocytes, Regulatory / cytology*
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B-Lymphocytes, Regulatory / immunology
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B7-H1 Antigen / biosynthesis*
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B7-H1 Antigen / physiology
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CD4-Positive T-Lymphocytes / cytology
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Cell Differentiation
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Cell Separation
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Cytokines / metabolism
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Encephalomyelitis, Autoimmune, Experimental / immunology
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Female
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Homeostasis
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Humans
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Immune System
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Immunity, Humoral / immunology*
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Immunoglobulin G / immunology
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Inflammation
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Interleukin-10 / metabolism
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Mice
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Mice, Inbred C57BL
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Programmed Cell Death 1 Receptor / metabolism*
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Receptors, CXCR5 / metabolism
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Young Adult
Substances
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Antibodies, Monoclonal
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B7-H1 Antigen
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CD274 protein, human
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CXCR5 protein, human
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CXCR5 protein, mouse
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Cd274 protein, mouse
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Cytokines
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Immunoglobulin G
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PDCD1 protein, human
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Pdcd1 protein, mouse
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Programmed Cell Death 1 Receptor
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Receptors, CXCR5
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Interleukin-10