The BH3-only protein Bad is dispensable for TNF-mediated cell death

Cell Death Dis. 2015 Jan 22;6(1):e1611. doi: 10.1038/cddis.2014.575.


Tumor necrosis factor (TNF) is a key signaling molecule orchestrating immune and inflammatory responses and possesses the capacity to trigger apoptotic as well as necroptotic cell death. Apoptotic cell death elicited by TNF has been demonstrated to engage pro-apoptotic Bcl-2 family proteins, most prominently the BH3-only protein Bid, a key substrate of caspase-8, the key effector protease downstream of TNF receptor I. Most recently, the BH3 domain-containing protein Bad (Bcl-2-antagonist of cell death) has been shown to be rate limiting for TNF-mediated cell death, suggesting possible synergy with Bid, but genetic analyses presented here demonstrate that it is dispensable for this process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Death / drug effects
  • Embryo, Mammalian / cytology
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Hepatitis / pathology
  • Hepatitis / prevention & control
  • Heterocyclic Compounds, 3-Ring / pharmacology
  • Humans
  • I-kappa B Kinase / antagonists & inhibitors
  • I-kappa B Kinase / metabolism
  • Mice, Inbred C57BL
  • Pyridines / pharmacology
  • Thymocytes / drug effects
  • Thymocytes / metabolism
  • Tumor Necrosis Factor-alpha / pharmacology*
  • bcl-Associated Death Protein / deficiency
  • bcl-Associated Death Protein / metabolism*


  • Heterocyclic Compounds, 3-Ring
  • PS1145
  • Pyridines
  • Tumor Necrosis Factor-alpha
  • bcl-Associated Death Protein
  • I-kappa B Kinase