Monitoring the distribution of surfactants in the stratum corneum by combined ATR-FTIR and tape-stripping experiments

Skin Pharmacol Physiol. 2015;28(3):167-75. doi: 10.1159/000368444. Epub 2015 Jan 21.

Abstract

Combined ATR-FTIR (attenuated total reflection-Fourier transform infrared) spectroscopy and tape-stripping experiments in vitro on porcine ear skin were used to investigate the spatial distribution of different surfactants in the stratum corneum (SC). To reveal a possible connection between the size of the formed micelles and skin penetration, dynamic light-scattering measurements of the aqueous surfactant solutions were also taken. Compared to an alkyl polyglycoside and sucrose laurate, a deeper skin penetration of the anionic surfactants sodium dodecyl sulfate (SDS) und sodium lauryl ether sulfate (SLES) could be related to a smaller size of the formed micelles. Beside the differences in spatial distribution, a link between the physical presence of anionic surfactants in the SC and a decrease of skin hydration was found. Furthermore, the incorporation of SDS and SLES into the SC, even after a brief, consumer-orientated washing procedure with commercially available hair shampoos, was confirmed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesives
  • Animals
  • Caco-2 Cells
  • Cell Survival / drug effects
  • Epidermis / drug effects
  • Epidermis / metabolism*
  • Glucans / pharmacology*
  • Hair Preparations
  • Humans
  • Micelles
  • Skin Absorption
  • Sodium Dodecyl Sulfate / analogs & derivatives*
  • Sodium Dodecyl Sulfate / pharmacology*
  • Spectroscopy, Fourier Transform Infrared / methods
  • Sucrose / analogs & derivatives*
  • Sucrose / pharmacology
  • Surface-Active Agents / pharmacology*
  • Swine

Substances

  • Adhesives
  • Glucans
  • Hair Preparations
  • Micelles
  • Plantacare 810
  • Surface-Active Agents
  • sucrose monolaurate
  • Sodium Dodecyl Sulfate
  • Sucrose
  • sodium laureth sulfate