Proteomics. Tissue-based Map of the Human Proteome

Science. 2015 Jan 23;347(6220):1260419. doi: 10.1126/science.1260419.

Abstract

Resolving the molecular details of proteome variation in the different tissues and organs of the human body will greatly increase our knowledge of human biology and disease. Here, we present a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray-based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level. Our tissue-based analysis detected more than 90% of the putative protein-coding genes. We used this approach to explore the human secretome, the membrane proteome, the druggable proteome, the cancer proteome, and the metabolic functions in 32 different tissues and organs. All the data are integrated in an interactive Web-based database that allows exploration of individual proteins, as well as navigation of global expression patterns, in all major tissues and organs in the human body.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Cell Line
  • Databases, Protein*
  • Female
  • Genes
  • Genetic Code
  • Humans
  • Internet
  • Male
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Mitochondrial Proteins / genetics
  • Mitochondrial Proteins / metabolism
  • Neoplasms / genetics
  • Neoplasms / metabolism
  • Protein Array Analysis
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • Proteome / genetics
  • Proteome / metabolism*
  • Tissue Distribution
  • Transcription, Genetic

Substances

  • Membrane Proteins
  • Mitochondrial Proteins
  • Protein Isoforms
  • Proteome