devR PCR for the diagnosis of intraocular tuberculosis

Ocul Immunol Inflamm. 2015 Feb;23(1):47-52. doi: 10.3109/09273948.2014.981550.


Purpose: To compare the efficacy of devR and MPB64 PCR in the diagnosis of intraocular tuberculosis.

Methods: Prospective, nonrandomized study. Seventy-five patients were enrolled in 3 groups. Group A had 25 patients with presumed intraocular tubercular uveitis, group B had 25 controls with specific uveitis other than tubercular uveitis, and group C included 25 non-uveitic negative controls. The undiluted vitreous/aqueous samples were collected and subjected to PCR assay for devR and MPB64 gene sequence of Mycobacterium tuberculosis (MTB) to detect sensitivity and specificity.

Results: devR PCR was positive in 16 (64%) out of 25 patients with presumed tubercular uveitis. MPB64 PCR was positive in 18 (72%) out of 25 patients with presumed tubercular uveitis. The sensitivity and specificity of devR were 64 and 100%, respectively. The sensitivity and specificity of MPB64 PCR were 72 and 100%, respectively.

Conclusion: devR PCR is not a better tool than MPB64 PCR for diagnosing intraocular tuberculosis.

Keywords: MPB64 PCR; devR PCR; polymerase chain reaction; retinal vasculitis; tubercular vasculitis.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aqueous Humor / virology
  • Child
  • DNA, Bacterial / analysis*
  • Diagnosis, Differential
  • Female
  • Follow-Up Studies
  • Humans
  • Male
  • Middle Aged
  • Mycobacterium tuberculosis / genetics*
  • Mycobacterium tuberculosis / isolation & purification
  • Polymerase Chain Reaction
  • Prospective Studies
  • Tuberculosis, Ocular / diagnosis*
  • Tuberculosis, Ocular / microbiology
  • Uveitis / diagnosis*
  • Uveitis / microbiology
  • Vitreous Body / virology
  • Young Adult


  • DNA, Bacterial