Full genome sequence analysis of a wild, non-MLV-related type 2 Hungarian PRRSV variant isolated in Europe

Gyula Balka; Xiong Wang; Ferenc Olasz; Ádám Bálint; István Kiss; Krisztián Bányai; Miklós Rusvai; Tomasz Stadejek; Douglas Marthaler; Michael P Murtaugh; Zoltán Zádori

doi:10.1016/j.virusres.2015.01.014

Full genome sequence analysis of a wild, non-MLV-related type 2 Hungarian PRRSV variant isolated in Europe

Virus Res. 2015 Mar 16:200:1-8. doi: 10.1016/j.virusres.2015.01.014. Epub 2015 Jan 20.

Authors

Affiliations

¹ Department of Pathology, Faculty of Veterinary Science, Szent István University, István u. 2, H-1078 Budapest, Hungary. Electronic address: balka.gyula@aotk.szie.hu.
² Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, USA.
³ Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, H-1143 Budapest, Hungary.
⁴ National Food Chain Safety Office Veterinary Diagnostic Directorate, Tábornok u. 2, H-1143 Budapest, Hungary.
⁵ Ceva-Phylaxia Veterinary Biologicals Co. Ltd., Szállás u. 5, H-1107 Budapest, Hungary.
⁶ Department of Pathology, Faculty of Veterinary Science, Szent István University, István u. 2, H-1078 Budapest, Hungary.
⁷ Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, ul. Nowoursynowska 159c, 02-776 Warsaw, Poland.
⁸ Veterinary Diagnostic Laboratory, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN 55108, USA.

PMID: 25616050
DOI: 10.1016/j.virusres.2015.01.014

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is a widespread pathogen of pigs causing significant economic losses to the swine industry. The expanding diversity of PRRSV strains makes the diagnosis, control and eradication of the disease more and more difficult. In the present study, the authors report the full genome sequencing of a type 2 PRRSV strain isolated from piglet carcasses in Hungary. Next generation sequencing was used to determine the complete genome sequence of the isolate (PRRSV-2/Hungary/102/2012). Recombination analysis performed with the available full-length genome sequences showed no evidence of such event with other known PRRSV. Unique deletions and an insertion were found in the nsp2 region of PRRSV-2/Hungary/102/2012 when it was compared to the highly virulent VR2332 and JXA-1 prototype strains. The majority of amino acid alterations in GP4 and GP5 of the virus were in the known antigenic regions suggesting an important role for immunological pressure in PRRSV-2/Hungary/102/2012 evolution. Phylogenetic analysis revealed that it belongs to lineage 1 or 2 of type 2 PRRSV. Considering the lack of related PRRSV in Europe, except for a partial sequence from Slovakia, the ancestor of PRRSV-2/Hungary/102/2012 was most probably transported from North-America. It is the first documented type 2 PRRSV isolated in Europe that is not related to the Ingelvac MLV.

Keywords: Full genome; Next generation sequencing; PRRSV; Phylogeny; Swine; Type 2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Europe
Genome, Viral*
Genotype
High-Throughput Nucleotide Sequencing
Hungary
Molecular Sequence Data
Phylogeny
Porcine Reproductive and Respiratory Syndrome / virology*
Porcine respiratory and reproductive syndrome virus / chemistry
Porcine respiratory and reproductive syndrome virus / classification
Porcine respiratory and reproductive syndrome virus / genetics
Porcine respiratory and reproductive syndrome virus / isolation & purification*
Swine
Viral Proteins / chemistry
Viral Proteins / genetics

Substances

Viral Proteins