Preventive treatment of calcium oxalate crystal deposition with immortal flowers

J Ethnopharmacol. 2015 Apr 2;163:60-7. doi: 10.1016/j.jep.2015.01.009. Epub 2015 Jan 21.


Ethnopharmacological relevance: A number of medicinal plants are used for their diuretic, urolithiatic and anti-inflammatory effects on urinary system problems in Turkey and the most common traditional remedy for kidney stones is the tea of immortal flowers. The aim of this study is to evaluate the preventive effect of infusions prepared from capitulums of Helichrysum graveolens (M.Bieb.) Sweet (HG) and Helichrysum stoechas ssp. barellieri (Ten.) Nyman (HS) on formation of kidney stones.

Materials and method: Sodium oxalate (Ox-70mg/kg intraperitoneally) was used to induce kidney stones on Wistar albino rats. At the same time, two different doses of the plant extracts (HG: 62.5 and 125mg/kg; HS: 78 and 156mg/kg) were dissolved in the drinking water and administered to animals for 5 days. Potassium citrate was used as positive control in the experiments. During the experiment, water intake, urine volume and body weights of the animals were recorded. At the end of the experiments, liver, kidney and body weights of the animals were determined; biochemical analysis were conducted on urine, blood and plasma samples. Histopathological changes in kidney tissues were examined and statistical analysis were evaluated.

Results: HS extract showed the highest preventive effect at 156mg/kg dose (stone formation score: 1.16), whereas a number of kidney stones were maximum in sodium oxalate group (stone formation score: 2.66). Helichrysum extracts decreased urine oxalate and uric acid levels and increased citrate levels significantly. In addition, Helichrysum extracts regulated the negative changes in biochemical and hematological parameters occurred after Ox injection.

Conclusions: We conclude that Helichrysum extracts could reduce the formation and growth of kidney stones in Ox-induced urolithiasis and can be beneficial for patients with recurrent stones. In addition, this is the first study on the preventive effect of immortal flowers.

Keywords: Asteraceae; Calcium oxalate crystallization; Helichrysum; Immortal flowers; Kidney stone; Urolithiasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Citrates / urine
  • Flowers
  • Helichrysum*
  • Kidney / drug effects
  • Kidney / pathology
  • Male
  • Nephrolithiasis / chemically induced
  • Nephrolithiasis / pathology
  • Nephrolithiasis / prevention & control*
  • Nephrolithiasis / urine
  • Oxalates / toxicity
  • Oxalates / urine
  • Phytotherapy*
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • Rats, Wistar
  • Uric Acid / urine


  • Citrates
  • Oxalates
  • Plant Extracts
  • Uric Acid

Supplementary concepts

  • Nephrolithiasis, Calcium Oxalate