Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Filters applied. Clear all
. 2015 Jan 24;11(1):e1004630.
doi: 10.1371/journal.ppat.1004630. eCollection 2015 Jan.

Cell Cycle-Independent Phospho-Regulation of Fkh2 During Hyphal Growth Regulates Candida Albicans Pathogenesis

Affiliations
Free PMC article

Cell Cycle-Independent Phospho-Regulation of Fkh2 During Hyphal Growth Regulates Candida Albicans Pathogenesis

Jamie A Greig et al. PLoS Pathog. .
Free PMC article

Abstract

The opportunistic human fungal pathogen, Candida albicans, undergoes morphological and transcriptional adaptation in the switch from commensalism to pathogenicity. Although previous gene-knockout studies have identified many factors involved in this transformation, it remains unclear how these factors are regulated to coordinate the switch. Investigating morphogenetic control by post-translational phosphorylation has generated important regulatory insights into this process, especially focusing on coordinated control by the cyclin-dependent kinase Cdc28. Here we have identified the Fkh2 transcription factor as a regulatory target of both Cdc28 and the cell wall biosynthesis kinase Cbk1, in a role distinct from its conserved function in cell cycle progression. In stationary phase yeast cells 2D gel electrophoresis shows that there is a diverse pool of Fkh2 phospho-isoforms. For a short window on hyphal induction, far before START in the cell cycle, the phosphorylation profile is transformed before reverting to the yeast profile. This transformation does not occur when stationary phase cells are reinoculated into fresh medium supporting yeast growth. Mass spectrometry and mutational analyses identified residues phosphorylated by Cdc28 and Cbk1. Substitution of these residues with non-phosphorylatable alanine altered the yeast phosphorylation profile and abrogated the characteristic transformation to the hyphal profile. Transcript profiling of the phosphorylation site mutant revealed that the hyphal phosphorylation profile is required for the expression of genes involved in pathogenesis, host interaction and biofilm formation. We confirmed that these changes in gene expression resulted in corresponding defects in pathogenic processes. Furthermore, we identified that Fkh2 interacts with the chromatin modifier Pob3 in a phosphorylation-dependent manner, thereby providing a possible mechanism by which the phosphorylation of Fkh2 regulates its specificity. Thus, we have discovered a novel cell cycle-independent phospho-regulatory event that subverts a key component of the cell cycle machinery to a role in the switch from commensalism to pathogenicity.

Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Fkh2 is differentially phosphorylated between yeast and hyphal growth.
A) Early G1 cells expressing Fkh2-YFP were collected by elutriation and re-inoculated into yeast growth conditions. Samples were taken for αGFP Western blot to observe Fkh2 phosphorylation and microscopy to follow cell cycle progression via budding and DAPI stained nuclei (n = 50) Note YFP is recognised by the αGFP monoclonal antibody; αCdc11 was used as a control for equal loading. B) Early G1 cells expressing Fkh2-YFP and Cdc12-mCherry were collected by elutriation and re-inoculated into hyphal growth conditions. Samples were taken as above, with cell cycle progression followed by monitoring septin ring formation and nuclear migration/division (n = 50). C) Confirmation of Fkh2 phosphorylation by phosphatase treatment. 80 min yeast and 40 min hyphae samples were taken and lysates treated at 30°C for 1 h with/without Lambda-phosphatase (NEB) and then resolved by 7% 1D PAGE. D) Fkh2 phosphorylation early on hyphal induction. Samples were taken at the indicated time points after hyphal induction and resolved by 1D PAGE as previously mentioned. In Figs. 1B–D αPSTAIRE was used as the loading control. E) Fkh2-YFP was isolated from cells in the culture conditions and times indicated and fractionated by 2D gel electrophoresis. Note the region of darkening at the acidic edge of the gel is where the sample was applied and does not come from Fkh2. An intensity profile is shown above each autoradiograph. In this and subsequent figures the profile was scaled to give maximum height to the maximum peak in the informative part of the gel. Where necessary some values from the non-specific part of the gel were omitted. Fig. 1E is shown with an independent replicate in S2 Fig.
Fig 2
Fig 2. Fkh2 is phosphorylated by Cdc28.
A) Schematic showing Cdc28 minimal (circles) and full (diamond) consensus target sites on Fkh2, with those detected by phospho-peptide mapping to be phosphorylated on hyphal induction indicated in blue. B) Phosphorylation of Fkh2 on hyphal induction detected using an antibody that recognises phosphorylated residues in Cdc28 target sites (αPSER(CDK) (Cell-Signalling 2324S). C) In vitro kinase assay with Cdc28-HA purified from a hyphal lysate and recombinant GST-Fkh2(CT) (aa419–687 intron removed) and GST-Fkh2-A-CT (as GST-Fkh2(CT) fragment with the serine/threonine in the five C-terminal Cdc28 consensus sites mutated to alanine). GST-Fkh2(CT) but not GST-Fkh2-A-CT is phosphorylated in vitro by Cdc28-HA. The parental strain BWP17, in which Cdc28 is not HA-tagged, provided the mock lysate to demonstrate that the activity was not due to a co-purifying kinase. D) Removal of Fkh2’s C-terminus containing the Cdc28 target site cluster abolishes the double band upon hyphal induction. fkh2(1–426)-GFP and fkh2/FKH2-YFP were grown as yeast or hyphae as previously, samples were treated with/without phosphatase and then resolved by SDS-PAGE. E) Autoradiograms from 2D gels and quantitative intensity profiles of the indicated Fkh2 phosphosite mutants at the indicated times and in the indicated culture conditions. The grey dashed line represents the parental Fkh2-YFP profile grown in the corresponding condition as shown in Fig. 1.
Fig 3
Fig 3. The effect of inhibiting Cdc28 or the removal of Cdc28 cyclins on Fkh2 phosphorylation.
Fkh2 from cells of the indicated genotype and culture condition was fractionated by 2D gels. The cdc28-as1 strain was treated with 30 µM 1NM-PP1 to inhibit Cdc28. Note it is not possible to grow cells to stationary phase with Cdc28 inhibited. The CLN3-sd strain was grown to stationary phase overnight in YEPD which allows partial de-repression of the MET3 promoter regulating CLN3 expression [76]. For hyphal and yeast growth cells were inoculated from these stationary phase cultures into YEPD medium containing 2.5 mM methionine and 0.5 mM cysteine to repress CLN3 expression.
Fig 4
Fig 4. Microarray analysis of Fkh2 mutants.
Microarray results sorted for the top 30 genes down/up regulated compared to the parental strain in the fkh2(6A) or fkh2ΔΔ mutants as indicated. Note the gene ranked 30 down regulated in the fkh2(6A) mutant (orf19.715 3-fold down) has been replaced with SUN41 (2-fold down; adjusted p-value = 5.4 × 10-5) as indicated by the dotted line. The full microarray of genes up or down regulated in all Fkh2 mutants is available in S2 Dataset. Gene annotation was provided from the Candida genome database http://www.candidagenome.org. Also shown is the presence of a predicted Fkh2 consensus (G/ATAAAC/TAAA) or minimal (AAAT/CAAA) binding site in the upstream 1 kb of each gene.
Fig 5
Fig 5. GO analysis of fkh2(6A) and fkh2ΔΔ mutants.
A) Venn diagram showing the number of genes down regulated in the fkh2(6A) and fkh2ΔΔ mutants and the overlap in the two data sets. B) Genes significantly more down regulated in fkh2ΔΔ mutant compared to the fkh2(6A) mutant and genes more down regulated in the fkh2(6A) mutant compared to fkh2ΔΔ. Each panel shows the genes down regulated at least two fold at the 5% FDR threshold in fkh2(6A) or fkh2ΔΔ (empirical Bayes moderated t-test). Genes significantly down regulated in fkh2ΔΔ (above) or fkh2(6A) (below) are highlighted in the solid points. Those genes significantly (5% FDR, empirical Bayes moderated t-tests) more down regulated in fkh2ΔΔ than fkh2(6A) are shown in blue (top panel), or more down regulated in fkh2(6A) than fkh2ΔΔ are shown in red (lower panel). Right: GO pathways enriched in genes in each category highlighted on the left. Note for reasons explained in the text the figures in this panel are not consistent with the Venn diagram shown in panel A. C) qPCR comparing the expression of hyphal associated transcripts in the Fkh2 phosphorylation mutants. Expression levels are normalised against ADE2 and shown relative to the expression in the fkh2/FKH2 strain. Means are from two independent biological repeats, each with three technical replicates. Vertical bars are equal to one standard error.
Fig 6
Fig 6. The effect of C-terminal deletion or phosphosite mutations on cell cycle progress during hyphal growth.
A) Stationary phase fkh2/FKH2 and fkh2(6A) cells (with a single nucleus) were inoculated into hyphal growth conditions. Samples were taken at 30 min intervals from 90 to 180 min, the hyphal cells were fixed with 1.5% formaldehyde and nuclei stained with DAPI. Hyphae were scored according to whether they had a single nucleus in the mother cell, a single migrating nucleus, two or three nuclei. A minimum of 100 cells were counted. B) Nuclear distribution of the indicated genotypes 180 min after hyphal induction. A minimum of 100 cells were counted. The distribution of nuclei in the fkh2∆∆ strain was so perturbed that it was not possible to make a meaningful assessment of nuclear content. C) Appearance of cells 180 min after hyphal induction. Images are shown as the merged DAPI (white) and DIC channels, and scale bars are equal to 10 μm. The DIC channel has been darkened for ease of nuclear visualisation.
Fig 7
Fig 7. Phosphorylation of Fkh2 affects the long-term maintenance of hyphal growth.
A) Phenotypes of Fkh2 phosphorylation mutants of the indicated genotypes. Left: grown as hyphae for 6 h in GMM with 10% FCS, fixed with 1.5% formaldehyde and pepsin treated. White arrows indicate branching and black arrows indicate constrictions. Right: yeast cells were re-inoculated into GMM pH 4.0 for 4hrs before formaldehyde fixation. Scale bars are equal to 10 μm. B) and C). Quantitation (n = 50) of the hyphal phenotypes was carried out to quantitate hyphal branching (B), and constrictions within a hypha (C). For Figs. B and C, a Z-test was used to compare the proportion of cells displaying the phenotype in the mutants with the wild type. * z < 0.05, ** z < 0.01. D) Long-term effects on hyphal growth observed on solid Spider medium. An overnight culture was diluted to OD600 = 1.0 and then serially diluted 10 fold as shown, with 1 μl of each dilution being spotted and the plates left at 37°C for five days. The extent of invasion was observed through washing off the surface colony using deionised water.
Fig 8
Fig 8. Biological confirmation of the microarray results—phosphorylation of Fkh2 affects multiple pathogenic processes.
A) Biofilm formation in 12 well plastic plates imaged after 48 h. B) Quantitation shows the average weight for ten individual biofilms. C) Epithelial cell damage measured by LDH release in a TR146 oral epithelial monolayer infection model. D) and E) Immune activation in the infection model was measured by IL-1α (D) or IL-1β (E) production. Error bars are standard deviations. In C-E FKH2/FKH2 represents an ARG4+ URA3+ BWP17 strain that has identical auxotrophic requirements to the other strains tested; FKH2/FKH2 represents a strain with 2 wild-type alleles of FKH2, one of which is C-terminally tagged with YFP and is prototrophic for URA3 only. B-E: Significance of the indicated comparisons was assessed by unpaired, one-tailed t-tests * p ≤ 0.05, ** p ≤ 0.01.
Fig 9
Fig 9. Phosphorylation of Fkh2 does not alter its localisation, but affects its interaction with the chromatin modifier Pob3.
A) Fkh2-YFP/GFP localisation in log phase yeast cells or 40 min after hyphal induction. Images are taken at x100 magnification. Scale bar, 5 μm. B) Co-IP experiments of Fkh2 with Pob3 and Srp1 from samples taken 40 min post hyphal induction. 2 mg of total protein was used for IP and 50 μg for the lysates. IP products were washed twice with lysis buffer containing 150 mM NaCl. C) Interaction of Pob3 with Fkh2 phospho-mutants, performed as above. The co-immunoprecipitation was quantified by normalising the αHA signal (Pob3) against the immunoprecipitated αGFP (Fkh2) for each IP, and then expressing this value as a fraction of the wild type value.
Fig 10
Fig 10. Fkh2 is phosphorylated at serine 533 by Cbk1-Mob2.
A) Autoradiograms from 2D gels and quantitative intensity profiles of the indicated strains at the indicated times and culture conditions. The grey dashed line represents the wild type Fkh2-YFP profile grown in the corresponding condition as shown in Fig. 1. B) In vitro kinase assay with Mob2-HA purified from a 40 min hyphal culture and E. coli expressed Fkh2 C-terminal fragment used in Fig. 2C. A BWP17 mock lysate controls for the possibility of a co-precipitating kinase from the αHA IP. C) Morphology of fkh2 S533A mutants. fkh2/FKH2 and fkh2/fkh2(S533A). Cells were re-inoculated from stationary phase cultures into either yeast or hyphal growth conditions, grown for 6 h and then fixed with 1.5% formaldehyde. Hyphal cells were pepsin treated to remove clumps before imaging at x100 magnification. Scale bars represent 10 μm.

Similar articles

See all similar articles

Cited by 5 articles

References

    1. Perlroth J, Choi B, Spellberg B (2007) Nosocomial fungal infections: epidemiology, diagnosis, and treatment. Medical Mycology 45: 321–346. 10.1080/13693780701218689 - DOI - PubMed
    1. Kullberg BJ, Filler SG (2002) Candidemia. In: Calderone RA, editors. Candida and Candidiasis. Washington DC: ASM Press; pp. 327–340.
    1. Runke M (2002) Skin and mucous infections. In: Calderone R, editors. Candida and Candidiasis. Washington: ASM Press; pp. 307–325.
    1. Sudbery PE, Gow NAR, Berman J (2004) The distinct morphogenic states of Candida albicans . Trends Microbiol 12: 317–324. 10.1016/j.tim.2004.05.008 - DOI - PubMed
    1. Sudbery PE (2011) Growth of Candida albicans hyphae. Nat Rev Microbiol 9: 737–748. 10.1038/nrmicro2636 - DOI - PubMed

Publication types

MeSH terms

Associated data

Feedback