Identification of Ancylostoma ceylanicum in children from a tribal community in Tamil Nadu, India using a semi-nested PCR-RFLP tool

Trans R Soc Trop Med Hyg. 2015 Apr;109(4):283-5. doi: 10.1093/trstmh/trv001. Epub 2015 Jan 24.

Abstract

Background: It is generally assumed that hookworm infections in humans are caused by Necator americanus and Ancylostoma duodenale. However, previous studies have also reported the presence of the animal hookworm A. ceylanicum in human stools.

Methods: We determined hookworm infections in children in a tribal community in Tamil Nadu, India, using a semi-nested PCR-RFLP approach.

Results: The results indicate that human species account for a majority of the hookworm infections (N. americanus 39/41 [95%]; A. duodenale 6/41 [15%]), whereas the animal hookworm A. ceylanicum only accounts for a minority of the infections (5%; 2/41).

Conclusions: The results emphasize the need to consider zoonotic ancylostomiasis while developing strategies to control hookworm infections.

Keywords: Ancylostoma ceylanicum; Ancylostoma duodenale; India; Necator americanus; Tamil Nadu; Zoonosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ancylostoma / genetics
  • Ancylostoma / isolation & purification*
  • Ancylostoma / pathogenicity
  • Ancylostomiasis / diagnosis*
  • Ancylostomiasis / epidemiology
  • Ancylostomiasis / prevention & control
  • Animals
  • Child
  • Child, Preschool
  • Disease Reservoirs
  • Dogs
  • Feces / parasitology*
  • Humans
  • India / epidemiology
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Species Specificity
  • Zoonoses / epidemiology
  • Zoonoses / parasitology