Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 22 (2), 169-74

CRISPR-Cas9-based Photoactivatable Transcription System


CRISPR-Cas9-based Photoactivatable Transcription System

Yuta Nihongaki et al. Chem Biol.


Targeted endogenous gene activation is necessary for understanding complex gene networks and has great potential in medical and industrial applications. The CRISPR-Cas system offers simple and powerful tools for this purpose. However, these CRISPR-Cas-based tools for activating user-defined genes are unable to offer precise temporal control of gene expression, despite the fact that many biological phenomena are regulated by highly dynamic patterns of gene expression. Here we created a light-inducible, user-defined, endogenous gene activation system based on CRISPR-Cas9. We demonstrated that this CRISPR-Cas9-based transcription system can allow rapid and reversible targeted gene activation by light. In addition, using this system, we have exemplified photoactivation of multiple user-defined endogenous genes in mammalian cells. The present CRISPR-Cas9-based transcription system offers simple and versatile approaches for precise endogenous gene activation in basic biological research and biotechnology applications.

Similar articles

  • CRISPR/Cas9 Immune System as a Tool for Genome Engineering
    M Hryhorowicz et al. Arch Immunol Ther Exp (Warsz) 65 (3), 233-240. PMID 27699445. - Review
    CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) adaptive immune systems constitute a bacterial defence against invading nucleic a …
  • Optical Control of CRISPR/Cas9 Gene Editing
    J Hemphill et al. J Am Chem Soc 137 (17), 5642-5. PMID 25905628.
    The CRISPR/Cas9 system has emerged as an important tool in biomedical research for a wide range of applications, with significant potential for genome engineering and gen …
  • Plant Genome Engineering in Full Bloom
    J Lozano-Juste et al. Trends Plant Sci 19 (5), 284-7. PMID 24674878.
    The recent development of tools for precise editing of user-specified sequences is rapidly changing the landscape for plant genetics and biotechnology. It is now possible …
  • Engineering Synthetic TALE and CRISPR/Cas9 Transcription Factors for Regulating Gene Expression
    AM Kabadi et al. Methods 69 (2), 188-97. PMID 25010559.
    Engineered DNA-binding proteins that can be targeted to specific sites in the genome to manipulate gene expression have enabled many advances in biomedical research. This …
  • Expanding the Biologist's Toolkit With CRISPR-Cas9
    SH Sternberg et al. Mol Cell 58 (4), 568-74. PMID 26000842. - Review
    Few discoveries transform a discipline overnight, but biologists today can manipulate cells in ways never possible before, thanks to a peculiar form of prokaryotic adapti …
See all similar articles

Cited by 71 PubMed Central articles

See all "Cited by" articles

Publication types


LinkOut - more resources